亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Expression of an Antimicrobial Peptide via the Chloroplast Genome to Control Phytopathogenic Bacteria and Fungi

生物 丁香假单胞菌 微生物学 大丽花黄萎病 烟草 叶绿体 孢子萌发 病菌 转基因作物 植物 转基因 基因 孢子 生物化学
作者
Gerald DeGray,Kanniah Rajasekaran,Franzine Smith,John C. Sanford,Henry Daniell
出处
期刊:Plant Physiology [Oxford University Press]
卷期号:127 (3): 852-862 被引量:296
标识
DOI:10.1104/pp.010233
摘要

Abstract The antimicrobial peptide MSI-99, an analog of magainin 2, was expressed via the chloroplast genome to obtain high levels of expression in transgenic tobacco (Nicotiana tabacum var. Petit Havana) plants. Polymerase chain reaction products and Southern blots confirmed integration of MSI-99 into the chloroplast genome and achievement of homoplasmy, whereas northern blots confirmed transcription. Contrary to previous predictions, accumulation of MSI-99 in transgenic chloroplasts did not affect normal growth and development of the transgenic plants. This may be due to differences in the lipid composition of plastid membranes compared with the membranes of susceptible target microbes. In vitro assays with protein extracts from T1 and T2 plants confirmed that MSI-99 was expressed at high levels to provide 88% (T1) and 96% (T2) inhibition of growth against Pseudomonas syringae pv tabaci, a major plant pathogen. When germinated in the absence of spectinomycin selection, leaf extracts from T2 generation plants showed 96% inhibition of growth against P. syringae pv tabaci. In addition, leaf extracts from transgenic plants (T1) inhibited the growth of pregerminated spores of three fungal species,Aspergillus flavus, Fusarium moniliforme, and Verticillium dahliae, by more than 95% compared with non-transformed control plant extracts. In planta assays with the bacterial pathogen P. syringae pv tabaciresulted in areas of necrosis around the point of inoculation in control leaves, whereas transformed leaves showed no signs of necrosis, demonstrating high-dose release of the peptide at the site of infection by chloroplast lysis. In planta assays with the fungal pathogen,Colletotrichum destructivum, showed necrotic anthracnose lesions in non-transformed control leaves, whereas transformed leaves showed no lesions. Genetically engineering crop plants for disease resistance via the chloroplast genome instead of the nuclear genome is desirable to achieve high levels of expression and to prevent pollen-mediated escape of transgenes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
3秒前
77完成签到,获得积分10
12秒前
15秒前
研友_VZG7GZ应助ZHY采纳,获得10
22秒前
加菲丰丰应助77采纳,获得30
23秒前
草木发布了新的文献求助10
27秒前
我是老大应助草木采纳,获得10
38秒前
40秒前
Omni完成签到,获得积分10
44秒前
gt完成签到 ,获得积分10
47秒前
俭朴蜜蜂完成签到 ,获得积分10
50秒前
1分钟前
哭泣灯泡完成签到,获得积分10
1分钟前
自然的南露完成签到 ,获得积分10
1分钟前
凡人丿完成签到 ,获得积分10
1分钟前
1分钟前
零度完成签到 ,获得积分10
1分钟前
1分钟前
秋风今是完成签到 ,获得积分10
1分钟前
1分钟前
葛力发布了新的文献求助10
1分钟前
1分钟前
脑洞疼应助葛力采纳,获得10
1分钟前
ZHY发布了新的文献求助10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
李健的小迷弟应助狮子座采纳,获得10
1分钟前
CC完成签到 ,获得积分10
1分钟前
剑指东方是为谁应助kydd采纳,获得10
2分钟前
清脆糖豆完成签到,获得积分10
2分钟前
小透明发布了新的文献求助30
2分钟前
乐乐应助ZHY采纳,获得10
2分钟前
2分钟前
科研通AI5应助jyy采纳,获得50
2分钟前
3分钟前
3分钟前
葛力发布了新的文献求助10
3分钟前
jyy发布了新的文献求助50
3分钟前
3分钟前
科研小白完成签到 ,获得积分10
3分钟前
CC关注了科研通微信公众号
3分钟前
高分求助中
All the Birds of the World 4000
Production Logging: Theoretical and Interpretive Elements 3000
Animal Physiology 2000
Les Mantodea de Guyane Insecta, Polyneoptera 2000
Am Rande der Geschichte : mein Leben in China / Ruth Weiss 1500
CENTRAL BOOKS: A BRIEF HISTORY 1939 TO 1999 by Dave Cope 1000
Machine Learning Methods in Geoscience 1000
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3736624
求助须知:如何正确求助?哪些是违规求助? 3280584
关于积分的说明 10020088
捐赠科研通 2997281
什么是DOI,文献DOI怎么找? 1644507
邀请新用户注册赠送积分活动 782041
科研通“疑难数据库(出版商)”最低求助积分说明 749648