生物
重组DNA
病毒学
人类免疫缺陷病毒(HIV)
群(周期表)
抗原
群体特异性抗原
慢病毒
A组
分子生物学
病毒性疾病
遗传学
基因
医学
内科学
化学
有机化学
作者
Robert K. Hickman,Ana Vallari,Alan Golden,J K Lund,John Hackett,Cathy Brennan,Sushil G. Devare
标识
DOI:10.1016/s0166-0934(98)00017-2
摘要
Recombinant antigens and peptides were used to develop an HIV slot immunoblot assay to confirm and differentiate infection by HIV-1 group M, HIV-1 group O or HIV-2. Recombinant antigens from the gag, pol or env regions of HIV-1 and HIV-2, in addition to synthetic peptides from the immunodominant region (IDR) of transmembrane proteins gp41 (HIV-1) or gp36 (HIV-2), were blotted on nitrocellulose strips and used as a substitute for competitive Western blots. Evaluation of a large number of samples (N=440) from various regions of the world, using the immunoblot, showed effective differentiation of HIV-1 group M, HIV-1 group O and HIV-2. The immunoblot identified correctly all (24/24) HIV-1 group O samples that were confirmed subsequently by PCR and sequence analysis. The immunoblot is a useful tool for identifying HIV-1 group O seropositive samples and has the potential to identify other serological HIV variants that may represent detection problems for HIV screening assays using HIV-1 group M subtype B reagents.
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