溶血磷脂酸
HT1080型
细胞迁移
癌症研究
细胞
基因敲除
细胞培养
焦点粘着
庆大霉素保护试验
运动性
细胞外基质
细胞生长
细胞生物学
化学
分子生物学
受体
生物
医学
内科学
生物化学
转移
癌症
遗传学
作者
Eriko Tanabe,Misaho Kitayoshi,Kyohei Yoshikawa,Ayano Shibata,Kanya Honoki,Nobuyuki Fukushima,Toshifumi Tsujiuchi
标识
DOI:10.3109/10799893.2012.738689
摘要
Lysophosphatidic acid (LPA) interacts with at least six G protein-coupled transmembrane LPA receptors (LPA1-LPA6). Recently, we have reported that LPA3 indicated opposite effects on cell migration, depending on the cell types. In the present study, to assess an involvement of LPA3 on cell migration of sarcoma cells, we generated LPA receptor-3 (LPAR3)-knockdown (HT1080-sh3 and HOS-sh3, respectively) cells from fibrosarcoma HT1080 and osteosarcoma HOS cells, and measured their cell migration abilities. In cell motility assay with a Cell Culture Insert, both LPAR3-knockdown cells showed significantly lower cell motile activities than control cells. Next, to investigate the effect of LPAR3-knockdown on invasion activity, which degraded the extracellular matrices, the Matrigel-coated filter was used. HT1080-sh3 cells showed significantly low invasive activity compared with control cells, while no invasive activity was found in HOS-sh3 cells. In gelatin zymography, no significant difference of matrix metalloproteinase (MMP)-2 and MMP-9 activities were detected in all cells. The results indicated that LPA3 acts as a positive regulator of cell motility and invasion in sarcoma cells, suggesting that LPA signaling pathway via LPA3 may be involved in the progression of sarcoma cells.
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