Effects of automated collection methods on semen quality and economic efficiency of boar semen production

A new fluorescent probe (TCF-AC) that contains 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) skeleton has been developed. Probe TCF-AC exhibits highly selective and sensitive detection toward Pd0 in EtOH/H2O (1:1, v/v, PBS 20 mM, pH = 7.4) solution with fluorescence “turn on” and colorimetric changes. The Pd0 detection by TCF-AC holds some advantages including good anti-interference ability, a relative large Stokes shift (>100 nm), and a low detection limit (7.05 × 10−7 M). Cell imaging studies demonstrate that TCF-AC is applicable to detect Pd0 in living HeLa cells.