The Reaction ofcyclo-Triphosphate with L-Valine

Abstract The reaction of cyclo-triphosphate(P3m) with l-valine in an aqueous solution was studied by means of anionexchange chromatography, paper chromatography, chemical analysis for phosphorus and valine, IR spectroscopy, and ninhydrin reaction. 1) P3m reacted with l-valine at pH 10–12 to form an unknown compound, the amount of which reached about 22% at pH 12 and about 9% at pH 10. This compound decomposed into orthophosphate(P1) and valine at a lower pH. 2) The unknown compound was proved to be a P1-derivative of valine, (Remark: Graphics omitted.), which has a P–N bond in its molecule. 3) In the reaction of P3m with l-valine at pH 10, in addition to the unknown compound, small amounts of tetra(P4) and pentaphosphate(P5) were produced. 4) The disappearance rate of P3m in this reaction was first-order, and its rate constants were 0.317 and 0.138 d−1 at pH 12 and 10 respectively at room temperature. 5) Short-chain phosphates, such as P1 pyro (P2), and triphosphate(P3), did not react with l-valine at all. 6) The mechanism of the reaction of P3m with l-valine was discussed.