Immune cell regulators in mouse mammary development and involution1

免疫系统 生物 细胞生物学 免疫学 细胞 遗传学
作者
Christine J. Watson
出处
期刊:Journal of Animal Science [Oxford University Press]
卷期号:87 (suppl_13): 35-42 被引量:33
标识
DOI:10.2527/jas.2008-1333
摘要

Dramatic changes in cell composition and function occur in the mammary gland during a pregnancy-lactation-involution cycle. We investigated the transcriptional changes associated with these biological events by using microarray analysis and identified the critical genes involved by using genetically modified mice. Two surprising findings arose from these studies. First, the microarray data showed that postlactational regression was associated with an acute phase inflammatory response, in addition to cell death. Conditional deletion of signal transducer and activator of transcription (Stat)3 or the nuclear factor-κB regulatory kinase inhibitor of κ B kinase β resulted in a failure of cell death induction during involution, an indication that these signaling pathways are essential mediators of the involution process. Both Stat3 and nuclear factor-κB have been shown to regulate acute phase gene expression in addition to apoptosis regulators. Four distinct transcriptional profiles are present in the first 4 d of involution, whereas there are 3 in lactation. At the peak of lactation (i.e., d 10 in mouse), more than 400 genes reach their maximum expression before declining dramatically in the first 12 h of involution. A reciprocal pattern was observed for more than 500 genes that were specifically upregulated within the first 12 h of forced involution. We are now investigating the role of a subset of these genes in involution. We also uncovered a role for genes normally associated with immune cell signaling in the differentiation of luminal mammary epithelial cells during pregnancy. Genetic deletion of the transcription factor Stat6 resulted in delayed development during pregnancy, and this phenotype was recapitulated in mammary tissue from IL-4 and IL-13 doubly deficient mice. Furthermore, we showed that mammary epithelial cells secrete T-cell regulatory cytokines. T-helper type 1 cytokines, such as interferon-γ and IL-12a, are secreted by undifferentiated mammary epithelial cells, whereas T-helper type 2 cytokines, including IL-4 and IL-13, are secreted by differentiated cells. This unexpected finding demonstrates a role for immune cell signaling in mammary epithelial cell fate and function.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
高大绝义发布了新的文献求助10
刚刚
爆米花应助drughunter009采纳,获得10
刚刚
正直的白羊完成签到 ,获得积分10
1秒前
Ivy完成签到,获得积分10
1秒前
杨tong完成签到 ,获得积分10
2秒前
4秒前
WerWu完成签到,获得积分10
4秒前
善良梦竹完成签到 ,获得积分10
4秒前
爱静静应助Mimi采纳,获得10
5秒前
耍酷的白山完成签到,获得积分10
5秒前
hao完成签到,获得积分10
6秒前
言树完成签到 ,获得积分10
6秒前
弃医遛鸟登高而歌完成签到 ,获得积分10
7秒前
kk子完成签到,获得积分10
7秒前
8秒前
小花小宝和阿飞完成签到 ,获得积分10
8秒前
ccboom完成签到,获得积分10
8秒前
犹豫小海豚完成签到,获得积分10
8秒前
大陆完成签到,获得积分10
9秒前
苻涵菡完成签到 ,获得积分10
10秒前
shy完成签到,获得积分10
10秒前
lily完成签到,获得积分10
12秒前
pcwang完成签到,获得积分10
14秒前
15秒前
无情的若枫完成签到,获得积分10
15秒前
极限地带完成签到 ,获得积分10
15秒前
15秒前
吃鱼的猫完成签到,获得积分10
16秒前
认真丹亦完成签到 ,获得积分10
17秒前
lijl0529完成签到,获得积分10
18秒前
川上富江完成签到 ,获得积分10
18秒前
知性的水杯完成签到 ,获得积分10
19秒前
柚又完成签到 ,获得积分10
19秒前
kankj发布了新的文献求助10
20秒前
biubiuu完成签到,获得积分10
22秒前
22秒前
沁沁完成签到,获得积分10
24秒前
JamesPei应助dan1029采纳,获得10
25秒前
香蕉觅云应助dan1029采纳,获得10
25秒前
汉堡包应助dan1029采纳,获得10
25秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 810
Pharmacogenomics: Applications to Patient Care, Third Edition 800
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3081634
求助须知:如何正确求助?哪些是违规求助? 2734500
关于积分的说明 7533221
捐赠科研通 2384096
什么是DOI,文献DOI怎么找? 1264167
科研通“疑难数据库(出版商)”最低求助积分说明 612567
版权声明 597584