Dose-dependent destruction of the coeruleus-cortical and nigral-striatal projections by MPTP

MPTP公司 神经科学 蓝斑 心理学 中枢神经系统 多巴胺 多巴胺能
作者
Nadine A. Seniuk,W. G. Tatton,Carol E. Greenwood
出处
期刊:Brain Research [Elsevier BV]
卷期号:527 (1): 7-20 被引量:151
标识
DOI:10.1016/0006-8993(90)91055-l
摘要

In order to determine whether 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) produces neuronal death or the loss of tyrosine hydroxylase (TH) immunoreactivity, 4 catecholaminergic nuclei in the mouse: substantia nigra compacta (SNc), locus coeruleus (LC), ventral tegmental area (VTA) and the A13 nucleus in the hypothalamus were quantitatively examined. Serial sections were taken through the restrocaudal extent of each nucleus: alternate sections were incubated with TH antiserum and reacted with an immunoperoxidase technique while the alternate set was Nissl stained. Counts and 3 dimensional reconstructions of TH reactive somata were made for each nucleus for saline-treated controls and mice treated with different doses of MPTP (37.5, 75, 150 and 300 mg/kg). TH-positive neurons were counted along with their counterparts on the Nissl-stained alternative sections to both identify the catecholaminergic neurons and to measure their destruction. Concentrations of striatal dopamine and cortical norepinephrine were measured for all dosages of MPTP in order to determine the relationship between dosage, target tissue neurotransmitter concentration and neuronal destruction. By 20 days after MPTP injection there was a dose-dependent random loss of TH-immunoreactive neurons that was almost identical in all 4 nuclei examined. Analysis of the Nissl versus TH cell counts revealed that MPTP resulted in neuronal destruction in the SNc and the LC rather than just a loss of TH immunoreactivity. There was no difference in sensitivity to MPTP between the SNc and the LC. Decreases in cortical norepinephrine concentrations were about one third of the decreases of LC neuronal counts for all MPTP doses; while decreases in striatal dopamine and SNc cell loss was similar to the LC for the two lower doses of MPTP but for the higher doses, the relationship approached or exceeded a one to one ratio. Hence estimates of neuronal death based upon target tissue transmitter concentrations could not be made using the same relationship for SNc and the LC catecholaminergic neurons and use of the same relationship for higher MPTP dosages results in an underestimate of LC neuronal destruction relative to that in the SNc.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
hh完成签到 ,获得积分10
刚刚
1秒前
缓慢如南应助yu采纳,获得10
1秒前
1秒前
鹿子轩发布了新的文献求助10
1秒前
1秒前
小Q啊啾发布了新的文献求助30
2秒前
2秒前
EddieDream发布了新的文献求助10
2秒前
乐乐应助滴滴采纳,获得10
3秒前
liyuxuan发布了新的文献求助10
3秒前
pluto应助zm采纳,获得10
3秒前
大模型应助子清采纳,获得10
3秒前
林一楠应助sullyeon采纳,获得10
3秒前
灵巧一笑发布了新的文献求助10
4秒前
5秒前
5秒前
椰叶发布了新的文献求助10
5秒前
6秒前
6秒前
欧皇降霖发布了新的文献求助10
6秒前
AA发布了新的文献求助10
7秒前
NexusExplorer应助arsenal采纳,获得10
7秒前
7秒前
sandman发布了新的文献求助10
7秒前
8秒前
8秒前
8秒前
没有发布了新的文献求助10
8秒前
七月流火应助shinn采纳,获得50
8秒前
Yy完成签到,获得积分10
9秒前
9秒前
9℃发布了新的文献求助10
10秒前
钟迪发布了新的文献求助10
10秒前
肖亚鑫发布了新的文献求助10
10秒前
10秒前
wxt完成签到,获得积分20
10秒前
ZeSir发布了新的文献求助10
11秒前
辛酸长安远啊完成签到 ,获得积分10
11秒前
syh发布了新的文献求助10
11秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3970949
求助须知:如何正确求助?哪些是违规求助? 3515634
关于积分的说明 11179061
捐赠科研通 3250769
什么是DOI,文献DOI怎么找? 1795474
邀请新用户注册赠送积分活动 875831
科研通“疑难数据库(出版商)”最低求助积分说明 805188