碱性成纤维细胞生长因子
再生(生物学)
细胞外基质
伤口愈合
成纤维细胞
静电纺丝
材料科学
体内
粘附
生物医学工程
生长因子
体外
解剖
细胞生物学
化学
外科
医学
内科学
生物
复合材料
生物化学
聚合物
受体
生物技术
作者
Ye Yang,Tian Xia,Wei Zhi,Wei Li,Jie Weng,Cong Zhang,Xiaohong Li
出处
期刊:Biomaterials
[Elsevier]
日期:2011-06-01
卷期号:32 (18): 4243-4254
被引量:328
标识
DOI:10.1016/j.biomaterials.2011.02.042
摘要
Diabetic skin ulcer is difficult to heal due to the lack of cellular and molecular signals required for normal wound repair. Emulsion electrospinning was adopted to imbed basic fibroblast growth factor (bFGF) into ultrafine fibers with a core-sheath structure to promote the wound healing process. An initially burst release as low as 14.0 ± 2.2% was achieved, followed by gradual release for around 4 wk. In vitro investigations on mouse embryo fibroblasts indicated that bFGF-loaded fibrous mats enhanced cell adhesion, proliferation, and secretion of extracellular matrix (ECM). Skin wounds were created in the dorsal area of diabetic rats for in vivo evaluation of skin regeneration after covered with bFGF-loaded fibrous mats. Compared with fibrous mats infiltrated with free bFGF, bFGF-loaded scaffolds revealed significantly higher wound recovery rate with complete re-epithelialization and regeneration of skin appendages. Higher density and mature capillary vessels were generated during 2 wk after treatment with bFGF-loaded fibers, and there was no fiber fragment observed in the histological sections at week 4 after operation. The gradual release of bFGF from fibrous mats enhanced collagen deposition and ECM remodeling, and the arrangement and component of collagen fibers were similar to normal tissues. The above results demonstrate the potential use of bFGF-loaded electrospun fibrous mats to rapidly restore the structural and functional properties of wounded skin for patients with diabetic mellitus.
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