Histopathological changes in tear-secreting tissues and cornea in a mouse model of autoimmune disease

角膜 泪腺 组织病理学 自身免疫性疾病 病理 医学 上皮 角膜上皮 内分泌学 内科学 眼科 疾病
作者
Masaya Hiraishi,Abdul Masum,Takashi Namba,Yuki Otani,Yaser Ha Elewa,Osamu Ichii,Yasuhiro Kon
出处
期刊:Experimental Biology and Medicine [SAGE Publishing]
卷期号:245 (12): 999-1008 被引量:3
标识
DOI:10.1177/1535370220928275
摘要

The tear film covers the cornea, and its abnormalities (including immunological) induce dry eye. Using autoimmune disease model mice, BXSB/MpJ- Yaa (BXSB-Yaa), histopathological changes in the eye and tear-secreting tissues were examined using histopathology, immunohistochemistry, and electron microscopy at 8, 20, and 28 weeks for early, middle, and late disease stages. Early and middle stage BXSB-Yaa showed increased serum autoantibody and spleen weight-to-body weight (S/B) ratio, respectively, and higher tear volume than controls, BXSB/MpJ (BXSB), at early stages, which decreased with ageing and negatively correlated with autoimmune disease indices. Smaller Meibomian gland acini, intraorbital lacrimal glands, and Harderian gland acinar cells were seen in late stage BXSB-Yaa than in BXSB; the latter two indices decreased with ageing and negatively correlated with the S/B ratio. Cell infiltration occurred in the middle stage BXSB-Yaa extraorbital lacrimal gland, and acinar cells were smaller than BXSB. The conjunctival goblet cells decreased from early to middle stages in both strains, but in BXSB-Yaa, they increased at late stages with a partial lack of microvilli on the cornea and were inversely altered with anterior epithelium thickness through ageing, suggesting that they compensated for anterior epithelium damage. In conclusion, the tear film was unstable due to an autoimmune disease condition in BXSB-Yaa. Impact statement Cornea, an outermost layer of mammalian eye, is protected by tear film and abnormalities of tear film causes dry eye. Dry eye injures the cornea which results lower vision in patients. Several factors cause dry eye, including altered systemic conditions, environment, and immunological abnormality of the patient in autoimmune disease like Sjögren’s syndrome (SS). However, the detailed pathology of autoimmune abnormality-mediated dry eye is unclear. Here we demonstrated that systemic autoimmune abnormality in BXSB-Yaa mice was associated with histological changes in the exocrine glands and cornea of the eyes. We also showed that BXSB-Yaa mice developed mild or early stage dry eye-like disease and explain the existence of a compensatory mechanism associated with the dysfunction of these tissues. Thus, BXSB-Yaa could be a model for SS-like disease-associated dry eye and these data would contribute to the understanding of the pathogenesis of autoimmune-related dry eye disease.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Zoe完成签到 ,获得积分10
1秒前
3秒前
星辰大海应助do0采纳,获得10
4秒前
tt完成签到 ,获得积分10
5秒前
浅辰完成签到,获得积分10
6秒前
大气萤完成签到,获得积分20
7秒前
7秒前
我ppp完成签到 ,获得积分10
7秒前
8秒前
易燃物品完成签到,获得积分10
8秒前
Hello应助Ther采纳,获得10
10秒前
CherylZhao完成签到,获得积分10
11秒前
Galato发布了新的文献求助10
12秒前
颜愫完成签到,获得积分10
12秒前
安详向日葵完成签到 ,获得积分10
13秒前
拼搏的白云完成签到,获得积分10
13秒前
852应助hhh采纳,获得10
13秒前
李白白白完成签到,获得积分10
13秒前
王手完成签到,获得积分10
13秒前
14秒前
一人完成签到,获得积分10
15秒前
do0完成签到,获得积分10
16秒前
yar应助xlz110采纳,获得10
16秒前
NexusExplorer应助落寞凌波采纳,获得10
18秒前
量子星尘发布了新的文献求助10
21秒前
123完成签到 ,获得积分10
21秒前
哈哈呵完成签到,获得积分10
21秒前
21秒前
Rylee完成签到,获得积分10
21秒前
Jiro完成签到,获得积分10
23秒前
我ppp发布了新的文献求助60
24秒前
25秒前
纳米酶催化完成签到,获得积分10
26秒前
26秒前
John完成签到,获得积分10
26秒前
李小强完成签到,获得积分10
27秒前
28秒前
31秒前
落寞凌波发布了新的文献求助10
31秒前
31秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029