In vitro Antioxidant activity of Exopolysaccharide extracted from Marine Sediment Soil bacteria

The aim of this study was identification and characterization of highly efficient Exopolysaccharide (EPS) producing bacteria in marine sediment soil. Molecular identification of this bacterium with the highest EPS production was carried out using 16S rRNA gene amplification. The results showed that this bacterium is belonging to the genus Bacillus. EPS productions were studied in different conditions (pH, temperature, carbon, nitrogen, incubation time, NaCl, metal ions and agricultural wastes) for the genus Bacillus. The production of EPS was observed highest while in the presence of pH-8, temperature 35, glucose, peptone, incubation time 24hrs, NaCl concentration 2%, calcium chloride and rice bran 84.16µg/ml. The in vitro antioxidant activity of exopolysaccharides such as hydrogen peroxide scavenging assay (81±0.14), DPPH radical scavenging assay (69±0.72), ABTS inhibition assay (64±0.34), hydroxyl scavenging assay (74±0.49), superoxide anion radical scavenging assay (71±0.27) were analysed by comparing with standard gallic acid. Bacterial EPS was analysed by FT-IR spectrum