赫拉
细胞凋亡
细胞周期
细胞周期检查点
细胞生长
多糖
周期素
MTT法
生物
细胞
流式细胞术
细胞周期蛋白
细胞生物学
化学
分子生物学
生物化学
作者
Jiao Xu,Zhicheng Tan,Zhongyuan Shen,Xiaoxu Shen,Shengnan Tang
标识
DOI:10.1016/j.fct.2021.111971
摘要
The present study presented the extraction and purification of polysaccharides from artificially cultured Cordyceps cicadae and wild Cordyceps cicadae by pre-soaking ultrasonic water extraction. The effects of different concentrations of polysaccharides on proliferation and cytotoxicity of Hela cells were detected by MTT and LDH methods. The results showed that the proliferation of Hela cells was inhibited by polysaccharides treatment (25 μg/mL-1600 μg/mL). The results of flow cytometry further confirmed that polysaccharides blocked the cell cycle in the S phase and promoted apoptosis. RT-qPCR and Western Blot were used to study the mRNA and protein expression of genes related to cell cycle and apoptosis signaling pathway. The results showed that polysaccharides treatment inhibited the expression of Cyclin E, Cyclin A and CDK2 and up regulated the expression of P53. Further, activation of Caspase cascade reaction, up regulation of death receptor, and the ratio of pro-apoptotic factor/anti-apoptotic factors, thus caused the cell cycle arrest and induced the apoptosis. The above research results lay a foundation for extending the anti-cancer effects of natural plant resources with low toxicity and high efficiency.
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