Simple methodology for ensuring the precision of measuring radioactivity at low concentrations in very small tissues using quantitative whole-body autoradiography

强度(物理) 相关系数 化学 统计 分析化学(期刊) 数学 物理 色谱法 光学
作者
Takanori Hironaka,Kazuhiro Inazawa,Yukari Tanaka,Hiroshi Hasegawa
出处
期刊:Regulatory Toxicology and Pharmacology [Elsevier BV]
卷期号:119: 104835-104835 被引量:1
标识
DOI:10.1016/j.yrtph.2020.104835
摘要

Quantitative whole-body autoradiography (QWBA) is largely used to evaluate tissue distribution of small molecule drugs. In QWBA, radioactivity is measured as the intensity obtained from the autoradiogram. It is known that lower intensity per a region of interest (ROI) or smaller size of ROI increases the variability of intensity. In fact, as some tissues are very small (e.g., the choroidea), ensuring reliability on the intensity for measuring radioactivity in these tissues is difficult in case of under- or over-estimation of radioactivity concentration owing to their variation of low radioactivity intensity of ROI. We thus analyzed the relationships between the size, intensity, and precision of ROI to determine the statistically significant lower limit of quantification (LLOQ) in very small tissues. To investigate the difference in correlation between the radiation source (commercial planar radiation standard [com-ST] and self-made radiation standard [self-ST] consisting of radioactive compounds and matrices), apparatus, or setting environment of the apparatus, correlation analysis was conducted under various conditions. Our results revealed that LLOQ can be calculated by simply using the correlation equation because a common relationship was observed between self-ST, which is used in QWBA, and com-ST. This methodology was thus considered valuable for ensuring LLOQ determination in QWBA. • We found a reliable and simple method for setting LLOQ in QWBA. • Accurate LLOQ can be set using this method with any apparatus at any facility. • In quantitative autoradiography, com-ST and self-ST shared common relationships.

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