分离(微生物学)
动物种类
生物
炎症
动物模型
细胞生物学
动物
免疫学
微生物学
内分泌学
作者
Daniel W. Siemsen,Liliya N. Kirpotina,Natalia Małachowa,Igor A. Schepetkin,Adeline R. Porter,Benfang Lei,Frank R. DeLeo,Mark T. Quinn
出处
期刊:Methods in molecular biology
日期:2019-11-15
卷期号:: 43-59
被引量:3
标识
DOI:10.1007/978-1-0716-0154-9_4
摘要
The development of new advances in understanding the role of neutrophils in inflammation requires effective procedures for isolating and purifying neutrophils. Methods for isolating human neutrophils are fairly standard, and some are covered in other chapters of this volume and previous editions. However, procedures for isolating neutrophils from nonhuman species used to model human diseases vary from those used in isolating human neutrophils and are not as well developed. Since neutrophils are highly reactive and sensitive to small perturbations, the methods of isolation are important to avoid isolation technique-induced alterations in cell function. We present methods here for reproducibly isolating highly purified neutrophils from large animal models (bovine, equine, ovine), small animal models (murine and rabbit), and nonhuman primates (cynomolgus macaques) and describe optimized details for obtaining the highest cell purity, yield, and viability.
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