谷氨酸棒杆菌
代谢工程
代谢途径
生物化学
底盘
生物合成
合成生物学
质粒
操纵子
焊剂(冶金)
化学
生物
基因
计算生物学
大肠杆菌
工程类
有机化学
结构工程
作者
Chenglin Zhang,Yanjun Li,Fuzhou Zhu,Zhixiang Li,Nan Lü,Yuhong Li,Qingyang Xu,Ning Chen
标识
DOI:10.1016/j.biortech.2020.124064
摘要
One challenge in metabolic engineering for industrial applications is the construction of highly efficient microbial cell factories. For this purpose, dynamic regulation of metabolic flux may be indispensable. In this study, an auto-regulated Corynebacterium glutamicum chassis for 5-aminolevulinic acid (5-ALA) biosynthesis was constructed. First, the expression of critical genes involved in 5-ALA synthesis and cofactor regeneration was precisely modulated. Furthermore, odhA expression was controlled using the strategies of static metabolic engineering (SME, with a weak promoter), dynamic metabolic engineering (DME, with a temperature-sensitive plasmid), and auto-inducible metabolic engineering (AME, with a growth-related promoter). The AME strategy showed the best effect and dynamically balanced the tradeoff between cell growth and 5-ALA synthesis. Additionally, the expression of exporter-encoding rhtA was regulated using AME strategy by the two-component system HrrSA in response to extracellular heme. The final strain A30 achieved the highest 5-ALA production (3.16 g/L) ever reported in C. glutamicum through C5 pathway.
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