Abstract 4144448: Evaluation of 3D Bioprinted Pulsatile Conduits Engineered Using Human Stem Cell-Derived Cardiomyocytes in a Murine Model

医学 脉动流 干细胞 电气导管 生物医学工程 组织工程 细胞生物学 心脏病学 机械工程 生物 工程类
作者
Syed Faaz Ashraf,Jacqueline M. Bliley,Tai Yi,Joseph Leland,Aram Kang,Maria Stang,Caner Dikyol,Anne Behre,Edith Tzeng,Christopher K. Breuer,Adam W. Feinberg
出处
期刊:Circulation [Lippincott Williams & Wilkins]
卷期号:150 (Suppl_1)
标识
DOI:10.1161/circ.150.suppl_1.4144448
摘要

Introduction: Patients with single ventricle disease and Fontan circulation suffer long-term morbidity due to the lack of a sub-pulmonary ventricle. The concept of a pulsatile Fontan conduit, functioning as a sub-pulmonary ‘neo-ventricle,’ using engineered heart tissue (EHT), may be a potential solution. To this end, we developed a tissue-engineered vascular conduit with EHT that allowed surgical implantation into a murine model for in-vivo assessment. Methods: Mouse-scale conduits were fabricated from type I collagen using freeform reversible embedding of suspended hydrogels (FRESH) 3D bioprinting. The conduits were cellularized using human stem cell-derived cardiomyocytes and cardiac fibroblasts. Electrophysiology was assessed using calcium imaging. The conduits were surgically anastomosed to the infra-renal inferior vena cava (IVC) as an end-end interposition graft in severe combined immunodeficiency (SCID)/beige mice. The conduit's patency and the EHT's contractility were monitored with monthly ultrasounds. Explanted conduits underwent histology, immunohistochemistry (IHC), and immunofluorescent (IF) imaging. Results: Sixteen contractile conduits were produced. In vitro calcium imaging demonstrated anisotropic calcium wave propagation along the conduit at day 14. Twelve grafts were implanted in the mouse IVC and half of the grafts (n=6) were explanted 6 months postoperatively. At explant, five showed synchronous contractile behavior in-vivo of the EHT around the collagen conduit. Interconnected networks of sarcomeric alpha actinin-positive cardiomyocytes were observed on IHC and IF. Neo-vascularization was seen infiltrating the EHT on histology. Six mice remained alive at 11 months post-operatively, three with contractile activity seen via ultrasound. All twelve grafts were patent on ultrasound with no stenosis. Conclusion: We have produced mouse-scale pulsatile conduits using human embryonic-derived cardiomyocytes and bioprinting that are robust enough to withstand surgical anastomosis to the IVC and remain patent. We observed functional beating of the EHT at 11 months in vivo. This model could be used to assess and improve the in-vivo function of EHT towards producing a larger-scale pulsatile Fontan conduit.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
自由的远侵完成签到 ,获得积分10
5秒前
巧克力手印完成签到,获得积分10
6秒前
屈岂愈发布了新的文献求助10
6秒前
7秒前
大橙子发布了新的文献求助10
8秒前
量子星尘发布了新的文献求助10
9秒前
jenny完成签到,获得积分10
10秒前
祁乐安完成签到,获得积分10
11秒前
naiyouqiu1989完成签到,获得积分10
12秒前
zjhzslq发布了新的文献求助10
13秒前
baoxiaozhai完成签到 ,获得积分10
14秒前
fy完成签到,获得积分10
15秒前
强公子完成签到,获得积分10
16秒前
22秒前
song完成签到 ,获得积分10
23秒前
怡然小蚂蚁完成签到 ,获得积分10
23秒前
小橙子完成签到,获得积分10
25秒前
SciGPT应助滴答采纳,获得10
25秒前
大气白翠完成签到,获得积分10
26秒前
确幸完成签到,获得积分10
26秒前
zjhzslq完成签到,获得积分10
26秒前
xdc发布了新的文献求助10
27秒前
ommphey完成签到 ,获得积分10
27秒前
牛哥还是强啊完成签到 ,获得积分10
28秒前
科研通AI2S应助屈岂愈采纳,获得10
29秒前
好名字完成签到,获得积分10
30秒前
kongzhiqiqi完成签到,获得积分10
31秒前
滴答完成签到 ,获得积分10
31秒前
浅浅殇完成签到,获得积分10
35秒前
36秒前
38秒前
滴答发布了新的文献求助10
41秒前
高高的天亦完成签到 ,获得积分10
42秒前
星空完成签到 ,获得积分10
43秒前
文艺的青旋完成签到 ,获得积分10
43秒前
青黛完成签到 ,获得积分10
50秒前
大橙子发布了新的文献求助10
54秒前
领导范儿应助科研通管家采纳,获得10
55秒前
量子星尘发布了新的文献求助10
59秒前
明钟达完成签到 ,获得积分10
1分钟前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038128
求助须知:如何正确求助?哪些是违规求助? 3575831
关于积分的说明 11373827
捐赠科研通 3305610
什么是DOI,文献DOI怎么找? 1819255
邀请新用户注册赠送积分活动 892655
科研通“疑难数据库(出版商)”最低求助积分说明 815022