Transcriptome and Metabolome Analyses Reveal Perfluorooctanoic Acid-Induced Kidney Injury by Interfering with PPAR Signaling Pathway

后代 转录组 超氧化物歧化酶 生物 氧化应激 内分泌学 内科学 代谢组 丙二醛 过氧化物酶体增殖物激活受体 生物化学 受体 代谢物 基因表达 怀孕 基因 医学 遗传学
作者
Yan Zhang,Li Yang,Nana Gao,Yinglan Gong,Wanyu Shi,Xiaodan Wang
出处
期刊:International Journal of Molecular Sciences [MDPI AG]
卷期号:24 (14): 11503-11503 被引量:8
标识
DOI:10.3390/ijms241411503
摘要

Perfluorooctanoic acid (PFOA) is widely used in aviation science and technology, transportation, electronics, kitchenware, and other household products. It is stable in the environment and has potential nephrotoxicity. To investigate the effect of PFOA exposure during pregnancy on the kidneys of offspring mice, a total of 20 mice at day 0 of gestation were randomly divided into two groups (10 mice in each group), and each group was administered 0.2 mL of PFOA at a dose of 3.5 mg/kg or deionized water by gavage during gestation. The kidney weight, kidney index, histopathological observation, serum biochemistry, transcriptomics, and metabolomics of the kidneys of the 35-day offspring mice were analyzed. In addition, malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) levels in the kidney were measured. Transcriptome analysis results showed that 387 genes were up-regulated and 283 genes were down-regulated compared with the control group. These differentially expressed genes (DEGs) were mainly concentrated in the peroxisome-proliferator-activated receptor (PPAR) signaling pathway and circadian rhythm. Compared with the control group, 64 and 73 metabolites were up- and down-regulated, respectively, in the PFOA group. The altered metabolites were mainly enriched in the biosynthesis of unsaturated fatty acids. PFOA can affect the expression levels of circadian rhythm-related genes in the kidneys of offspring mice, and this change is influenced by the PPAR signaling pathway. PFOA causes oxidative stress in the kidneys, which is responsible for significant changes in metabolites associated with the biosynthesis of unsaturated fatty acids.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Hjd完成签到,获得积分10
3秒前
伶俐的语雪完成签到,获得积分10
6秒前
8秒前
Splaink完成签到 ,获得积分10
10秒前
心想事成完成签到 ,获得积分10
13秒前
追忆发布了新的文献求助10
13秒前
刘雨森完成签到,获得积分10
13秒前
了凡完成签到 ,获得积分10
14秒前
丘比特应助Wang采纳,获得10
15秒前
xinxiangshicheng完成签到 ,获得积分10
17秒前
跳跃太清完成签到 ,获得积分10
22秒前
晨珂完成签到,获得积分10
22秒前
可靠的毛巾完成签到 ,获得积分10
26秒前
研友_GZ3zRn完成签到 ,获得积分0
36秒前
邵123456789完成签到,获得积分10
37秒前
科研狗完成签到 ,获得积分10
40秒前
sss完成签到 ,获得积分10
42秒前
YZ完成签到 ,获得积分10
47秒前
涂涂完成签到 ,获得积分10
53秒前
蓝意完成签到,获得积分0
1分钟前
Serein完成签到,获得积分10
1分钟前
celia完成签到 ,获得积分10
1分钟前
牛奶面包完成签到 ,获得积分10
1分钟前
桃儿完成签到 ,获得积分10
1分钟前
扬帆起航完成签到 ,获得积分10
1分钟前
开放又亦完成签到 ,获得积分10
1分钟前
依依完成签到,获得积分10
1分钟前
mc1220完成签到,获得积分10
1分钟前
czj完成签到 ,获得积分10
1分钟前
kuyi完成签到 ,获得积分10
1分钟前
像猫的狗完成签到 ,获得积分10
2分钟前
Julien完成签到,获得积分10
2分钟前
沉静香氛完成签到 ,获得积分10
2分钟前
尼可刹米洛贝林完成签到,获得积分10
2分钟前
刘荻萩完成签到 ,获得积分10
2分钟前
今后应助狼来了aas采纳,获得10
2分钟前
x银河里完成签到 ,获得积分10
2分钟前
a46539749完成签到 ,获得积分10
2分钟前
一一完成签到,获得积分10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
Typology of Conditional Constructions 500
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3571332
求助须知:如何正确求助?哪些是违规求助? 3141926
关于积分的说明 9444874
捐赠科研通 2843331
什么是DOI,文献DOI怎么找? 1562830
邀请新用户注册赠送积分活动 731326
科研通“疑难数据库(出版商)”最低求助积分说明 718524