纳米器件
适体
免疫检查点
免疫系统
癌症研究
分子成像
体内
纳米技术
生物
材料科学
免疫疗法
免疫学
分子生物学
生物技术
作者
Zheng Liu,Duan Xiang,Yangfang Yun,Siqi Li,Zhiyuan Feng,Jiayin Zhan,Ran Liu,Yan Li,Jingjing Zhang
出处
期刊:ACS Nano
[American Chemical Society]
日期:2024-01-19
被引量:1
标识
DOI:10.1021/acsnano.3c12499
摘要
Real-time dynamic imaging of immunoactivation-related cytokines is crucial for evaluating the efficacy of immune checkpoint blockade therapy and optimizing the treatment regimen. We introduce herein a spatiotemporally controlled nanodevice that allows in situ photoactivated imaging of interferon-gamma (IFN-γ) secretion from T cells in vitro and in vivo. The nanodevice is constructed by rational engineering of an aptamer-embedded, UV-cleavable PC-DNA probe and further integration with upconversion nanoparticles- and CRISPR-Cas12a-enhanced fluorescence systems. Using human peripheral blood mononuclear cells (PBMC)-engrafted mouse models, this nanodevice allows for the quantitative imaging of endogenous IFN-γ and its intratumoral dynamics responding to antiprogrammed cell death receptor 1 (anti-PD-1) therapy. This study thus provides a toolbox for boosting the sensitivity and precision of cytokine imaging during immune checkpoint blockade therapy, enlightening research toward imaging-guided tumor therapy.
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