Tuning the Luminescence of Microwave-Assisted N-Doped Fluorescent Carbon Dots: Bioimaging Applications and Label-Free Anti-Cancer Drug Delivery

Nanosized fluorescent carbon dots (Cdots) have gained a lot of attention in the recent years because of their superior properties, such as good biocompatibility, low toxicity, excellent chemical stability, resistance to photobleaching, and ease of chemical modification. Cdots are promising candidates for considerable applications in various fields: sensors, bioimaging, and drug delivery. Specifically, nitrogen-doped Cdots have attracted a huge interest because of their applicability in bioimaging and drug delivery. Conventional methods for the synthesis of Cdots have drawbacks, such as the use of organic solvents, the presence of side products, and the time required for synthesis. Keeping all these points in mind, herein, we report green methodology for the synthesis of water-soluble, blue-emitting, nitrogen-doped multifunctional Cdots under microwave irradiation within 3 min. The Cdots were prepared using citric acid and arginine as source materials and were characterized using various physicochemical techniques. A pH-responsive drug delivery system was then designed using anticancer drug doxorubicin and the synthesized Cdots. The biocompatibility of synthesized Cdots was analyzed against L929 normal cell line. The Cdots-DOX conjugates exhibited efficient anticancer activity against HeLa cells and also acted as excellent bioimaging agents.