清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Prognostic Value and Genome Signature of m6A/m5C Regulated Genes in Early-Stage Lung Adenocarcinoma

腺癌 基因 基因组 阶段(地层学) 生物 基因签名 价值(数学) 病理 癌症研究 遗传学 内科学 医学 基因表达 癌症 计算机科学 古生物学 机器学习
作者
Long Tian,Yan Wang,Jie Tian,Wenpeng Song,Lu Li,Guowei Che
出处
期刊:International Journal of Molecular Sciences [Multidisciplinary Digital Publishing Institute]
卷期号:24 (7): 6520-6520 被引量:6
标识
DOI:10.3390/ijms24076520
摘要

RNA modifications implicate pathological and prognosis significance in cancer development and progression, of which, m6A and m5C are representative regulators. These RNA modifications could produce effects on the function of other RNA by regulating gene expression. Thus, in this study, we aimed to explore the correlation between m6A/m5C regulators and early-stage lung adenocarcinoma (LUAD). Only the early-stage LUAD samples were included in this investigation, and the RNA-seq dataset of The Cancer Genome Atlas (TCGA) cohort was utilized to evaluate the expression of 37 m6A/m5C regulated genes. Based on the expression level of these 37 genes, early-stage LUAD patients were divided into 2 clusters, which were performed by consensus clustering, and the m6A/m5C subtypes had significantly different prognostic outcomes (p < 0.001). Cluster1, which has a better prognosis, was characterized by the C3 (inflammatory) immune subtype, low immune infiltration, chemokine expression, major histocompatibility complex (MHC) expression, and immune checkpoint molecule expression. Furthermore, compared with cluster1, cluster2 showed a T cell exhaustion state, characterized by a high expression of immune checkpoint genes, and immune cells, such as T cells, CD8+ T cells, cytotoxic lymphocytes, NK cells, and so on. In addition, patients in cluster2 were with high tumor mutational burden (TMB) and numerous significant mutated oncogene and tumor suppressor genes, such as WNT10B, ERBB4, SMARCA4, TP53, and CDKN2A (p < 0.001). A total of 19 genes were mostly related to the prognosis of LUAD and were upregulated in cluster2 (p < 0.05), showing a positive correlation with the mRNA expression of 37 m6A/m5C regulated genes. The predictive risk model was constructed using Cox and LASSO (least absolute shrinkage and selection operator) regression analysis. Finally, a seven-gene m6A/m5C risk model, comprising of METTL3, NPLOC4, RBM15, YTHDF1, IGF2BP1, NSUN3, and NSUN7, was constructed to stratify the prognosis of early-stage LUAD (p = 0.0049, AUC = 0.791). The high-risk score was associated with a poorer prognosis. This model was also validated using two additional GEO datasets: GSE72094 (p = 0.011, AUC = 0.736) and GSE50081 (p = 0.012, AUC = 0.628). In summary, it was established that the m6A/m5C-regulated genes performed a crosstalk function in the mRNA expression of early-stage LUAD. By interacting with other mRNA genes, m6A/m5C modification disturbs DNA replication and the tumor immune microenvironment (TIME). The seven-gene risk model may be a critical tool for the prognostic assessment of early-stage LUAD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
脑洞疼应助科研通管家采纳,获得10
2秒前
顾矜应助科研通管家采纳,获得10
2秒前
wjx完成签到 ,获得积分10
3秒前
John完成签到,获得积分10
14秒前
顾矜应助ceeray23采纳,获得20
25秒前
57秒前
Able完成签到,获得积分10
58秒前
我是笨蛋完成签到 ,获得积分10
1分钟前
ceeray23发布了新的文献求助20
1分钟前
Owen应助董可以采纳,获得10
1分钟前
酷酷妙梦完成签到,获得积分10
1分钟前
科研通AI2S应助彦嘉采纳,获得10
1分钟前
小二郎应助科研通管家采纳,获得10
2分钟前
hhh2018687完成签到,获得积分10
2分钟前
科研通AI2S应助ceeray23采纳,获得20
2分钟前
忘忧Aquarius完成签到,获得积分10
2分钟前
wujiwuhui完成签到 ,获得积分10
2分钟前
Lny发布了新的文献求助30
2分钟前
sh1ro完成签到,获得积分10
2分钟前
luang应助ceeray23采纳,获得40
2分钟前
3分钟前
ww完成签到,获得积分10
3分钟前
斯文败类应助ceeray23采纳,获得20
3分钟前
机智秋莲发布了新的文献求助20
3分钟前
ChatGPT完成签到,获得积分10
3分钟前
所所应助科研通管家采纳,获得10
4分钟前
4分钟前
4分钟前
ceeray23发布了新的文献求助20
4分钟前
海阔天空完成签到 ,获得积分10
4分钟前
zys发布了新的文献求助10
4分钟前
ffdhdh应助LYZSh采纳,获得10
5分钟前
5分钟前
机智秋莲完成签到,获得积分20
5分钟前
欣欣子完成签到 ,获得积分10
5分钟前
apt完成签到 ,获得积分10
5分钟前
科研通AI2S应助科研通管家采纳,获得10
6分钟前
6分钟前
董可以发布了新的文献求助10
7分钟前
Orange应助董可以采纳,获得10
7分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990543
求助须知:如何正确求助?哪些是违规求助? 3532220
关于积分的说明 11256532
捐赠科研通 3271057
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234