CD16
生物
Fc受体
Janus激酶3
白细胞介素21
白细胞介素12
抗体
淋巴因子激活杀伤细胞
细胞生物学
免疫学
自然杀伤细胞
受体
NK-92
先天免疫系统
CD49b
免疫系统
T细胞
体外
CD3型
细胞毒性T细胞
CD8型
生物化学
作者
Oscar A. Aguilar,Maria D. R. Gonzalez-Hinojosa,Janice Arakawa-Hoyt,Alberto J. Millan,Dagmar Gotthardt,Tsukasa Nabekura,Lewis L. Lanier
标识
DOI:10.1093/jleuko/qiac003
摘要
Abstract Natural killer (NK) cells are innate lymphocytes capable of mediating immune responses without prior sensitization. NK cells express Fc-gamma receptors (FcγRs) that engage the Fc region of IgG. Studies investigating the role of FcγRs on mouse NK cells have been limited due to lack specific reagents. In this study, we characterize the expression and biological consequences of activating mouse NK cells through their FcγRs. We demonstrate that most NK cells express the activating CD16 receptor, and a subset of NK cells also expresses the inhibitory CD32b receptor. Critically, these FcγRs are functional on mouse NK cells and can modulate antibody-mediated responses. We also characterized mice with conditional knockout alleles of Fcgr3 (CD16) or Fcgr2b (CD32b) in the NK and innate lymphoid cell (ILC) lineage. NK cells in these mice did not reveal any developmental defects and were responsive to cross-linking activating NK receptors, cytokine stimulation, and killing of YAC-1 targets. Importantly, CD16-deficient NK cells failed to induce antibody-directed cellular cytotoxicity of antibody-coated B-cell lymphomas in in vitro assays. In addition, we demonstrate the important role of CD16 on NK cells using an in vivo model of cancer immunotherapy using anti-CD20 antibody treatment of B-cell lymphomas.
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