Quercetin‐loaded sodium alginate/collagen/h‐boron nitride potential wound dressings prepared using the Box‐Behnken experimental design

Abstract Background/Aims Natural and synthetic biocompatible polymers have received significant attention in the pharmaceutical industry due to their rapid and effective healing properties in the wound healing process. The aim of this study was to optimize the extraction of onions, the preparation of sodium alginate/collagen/hydrogen boron nitride (NaAlg/Col/h‐BN) membranes using the Box‐Behnken experimental design, and determine the optimal conditions for quercetin release. The study also aimed to investigate the antimicrobial and antioxidant activities of the prepared membranes and their therapeutic properties. Methods and Results The prepared membranes were characterized by scanning electron microscopy (SEM), fourier transform infrared (FTIR), differential scanning calorimetry (DSC), and X‐ray diffraction (XRD). Antimicrobial activities were tested against Gram‐negative (Gr‐) Escherichia coli ATCC 25922, Klebsiella pneumonia , Enterobacter aerogenes , Gram‐positive (Gr+) Staphylococcus aureus ATCC 25923, and Candida albicans ATCC 10231 pathogens. In vitro release studies were conducted to examine the therapeutic properties of the prepared membranes. The optimum conditions for the extraction of onions and the preparation of NaAlg/Col/h‐BN membranes were found to be EtOH = 75 mL, t = 2 h, T = 45°C, and NaAlg = 1.0 g, Col = 2.0 g, and h‐BN = 6% wt, respectively. The prepared membranes exhibited serious antimicrobial properties against S. aureus and C. albicans . The membranes also promoted the controlled release of quercetin for 24 h in vitro, indicating their potential as a new approach in wound treatment. Conclusion The study concludes that quercetin‐filled NaAlg/Col/h‐BN membranes have promising therapeutic properties for wound healing. The membranes exhibited significant antimicrobial and antioxidant properties, and their controlled release of quercetin suggests their potential for use in wound healing applications.