A highly sensitive fluorescence nanosensor for determination of amikacin antibiotics using composites of carbon quantum dots and gold nanoparticles

Amikacin is an aminoglycoside antibiotic widely used to treat various bacterial infections in humans. However, elevated concentrations of amikacin can damage the cochlear nerve. Thus, accurate and rapid amikacin detection is crucial. In this study, we developed an "on-off" fluorescence nanosensor for highly sensitive amikacin determination based on a composite of carbon quantum dots (CQDs) and gold nanoparticles (AuNPs). The method quenches CQD fluorescence (turn-off) when they bind to AuNPs but restores it (turn-on) when amikacin binds and releases the CQDs. Adding Cu2+ enhances sensitivity by cross-linking amikacin-coated AuNPs. Under optimal conditions (pH 4, 1 mM Na2SO4, 1 mM CuSO4), the method achieved a low detection limit of 3.5 × 10-11 M (0.02 ppb), a wide linear range (10-10 to 10-8 M), high precision (RSD < 5 %), and a rapid 2-minute response time. Exceptional selectivity was observed over other antibiotics. The CQDs/AuNPs-based sensor successfully detected amikacin in pharmaceutical and surface water samples. This approach offers a fast on-site analytical method for amikacin detection, with potential applications in clinical and environmental settings.