转录组
表型
免疫学
单核细胞
炎症
生物
外周血
外围设备
红斑狼疮
细胞周期
细胞
医学
基因表达
基因
抗体
遗传学
内科学
作者
Eirini Maria Stergioti,Theodora Manolakou,George Sentis,Martina Samiotaki,Noemin Kapsala,Antonis Fanouriakis,Dimitrios T. Boumpas,Aggelos Banos
标识
DOI:10.1016/j.clim.2023.109765
摘要
Peripheral blood monocytes propagate inflammation in systemic lupus erythematosus (SLE). Three major populations of monocytes have been recognized namely classical (CM), intermediate (IM) and non-classical monocytes (NCM). Herein, we performed a comprehensive transcriptomic, proteomic and functional characterization of the three peripheral monocytic subsets from active SLE patients and healthy individuals. Our data demonstrate extensive molecular disruptions in circulating SLE NCM, characterized by enhanced inflammatory features such as deregulated DNA repair, cell cycle and heightened IFN signaling combined with differentiation and developmental cues. Enhanced DNA damage, elevated expression of p53, G0 arrest of cell cycle and increased autophagy stress the differentiation potential of NCM in SLE. This immunogenic profile is associated with an activated macrophage phenotype of NCM exhibiting M1 characteristics in the circulation, fueling the inflammatory response. Together, these findings identify circulating SLE NCM as a pathogenic cell type in the disease that could represent an additional therapeutic target.
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