BCA101 Is a Tumor-Targeted Bifunctional Fusion Antibody That Simultaneously Inhibits EGFR and TGFβ Signaling to Durably Suppress Tumor Growth

西妥昔单抗 癌症研究 抗体 化学 癌变 癌症 单克隆抗体 医学 免疫学 内科学
作者
Srinivas Reddy Boreddy,Reshmi Nair,Prashant Kumar Pandey,Anshu Kuriakose,Shivakumar Bhadravathi Marigowda,Chaitali Dey,Arindam Banerjee,Hanumant Kulkarni,Milind Sagar,Shiv Ram Krishn,Shruthi Rao,Madhukara AR,Vinita Tiwari,Bhavna Alke,Prashantha Kumar MV,Meena Shri,Chaitrali Dhamne,Sonal Patel,Pinky Sharma,Sankar Periyasamy,Jaya Bhatnagar,Moni Abraham Kuriakose,Ram Bhupal Reddy,Amritha Suresh,Suma Sreenivas,Nagaraja Govindappa,Praveen Reddy Moole,Usha Bughani,Seng-Lai Tan,Pradip Nair
出处
期刊:Cancer Research [American Association for Cancer Research]
卷期号:83 (11): 1883-1904 被引量:14
标识
DOI:10.1158/0008-5472.can-21-4425
摘要

The EGFR and TGFβ signaling pathways are important mediators of tumorigenesis, and cross-talk between them contributes to cancer progression and drug resistance. Therapies capable of simultaneously targeting EGFR and TGFβ could help improve patient outcomes across various cancer types. Here, we developed BCA101, an anti-EGFR IgG1 mAb linked to an extracellular domain of human TGFβRII. The TGFβ "trap" fused to the light chain in BCA101 did not sterically interfere with its ability to bind EGFR, inhibit cell proliferation, or mediate antibody-dependent cellular cytotoxicity. Functional neutralization of TGFβ by BCA101 was demonstrated by several in vitro assays. BCA101 increased production of proinflammatory cytokines and key markers associated with T-cell and natural killer-cell activation, while suppressing VEGF secretion. In addition, BCA101 inhibited differentiation of naïve CD4+ T cells to inducible regulatory T cells (iTreg) more strongly than the anti-EGFR antibody cetuximab. BCA101 localized to tumor tissues in xenograft mouse models with comparable kinetics to cetuximab, both having better tumor tissue retention over TGFβ "trap." TGFβ in tumors was neutralized by approximately 90% in animals dosed with 10 mg/kg of BCA101 compared with 54% in animals dosed with equimolar TGFβRII-Fc. In patient-derived xenograft mouse models of head and neck squamous cell carcinoma, BCA101 showed durable response after dose cessation. The combination of BCA101 and anti-PD1 antibody improved tumor inhibition in both B16-hEGFR-expressing syngeneic mouse models and in humanized HuNOG-EXL mice bearing human PC-3 xenografts. Together, these results support the clinical development of BCA101 as a monotherapy and in combination with immune checkpoint therapy.The bifunctional mAb fusion design of BCA101 targets it to the tumor microenvironment where it inhibits EGFR and neutralizes TGFβ to induce immune activation and to suppress tumor growth.
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