Oxidase-like nanozymes-driven colorimetric, fluorescence and electrochemiluminescence assays for pesticide residues

Pesticides are recognized as effective tools for crop protection, but pesticide residues pose a potential threat to human health and ecosystems. When pesticides are present in crops, they can enter the food chain and cause harm to humans and other organisms. Therefore, the monitoring of pesticide levels in relevant matrices is essential to ensure the safety of humans and other organisms. This paper reviews the applications of oxidase-like nanozymes-driven colorimetric, fluorescence and ECL assays for pesticide residues. The mechanisms of fluorescence, colorimetric and ECL assays are also presented. Finally, the advantages and disadvantages of colorimetric, fluorescence and ECL assays and traditional methods in pesticide detection were compared, and their applications in different pesticides detection were validated. This study aims to explore the feasibility and superiority of oxidase-like nanozymes-driven colorimetric, fluorescence and ECL assays for pesticide residues, and to provide an economical, sensitive and accurate detection method for pesticide residue detection. Oxidase-like nanozymes-driven colorimetric, fluorescence and ECL assays can rapidly and accurately detect the presence of pesticides under laboratory conditions with high accuracy and stability. Future research will focus on the development of portable assay equipment based on oxidase-like nanozymes to achieve rapid assay of pesticide residues in the field, which is of great significance in ensuring food safety and thus maintaining the health of humans and other organisms.