胎儿血红蛋白
色谱法
糖化血红素
毛细管电泳
血红蛋白
血红蛋白变体
免疫分析
罗氏诊断公司
分析物
化学
血红蛋白A
医学
内科学
糖尿病
免疫学
内分泌学
生物
2型糖尿病
胎儿
怀孕
遗传学
抗体
作者
Mingyang Li,Ge Song,Xin Shu,Xiongjun Wu,Haiyan Liu,Anping Xu,Ling Ji
出处
期刊:Labmedicine
[Oxford University Press]
日期:2024-05-27
卷期号:55 (6): 708-712
被引量:1
标识
DOI:10.1093/labmed/lmae034
摘要
Abstract Background Glycated hemoglobin, or hemoglobin A1c (HbA1c), serves as a crucial marker for diagnosing diabetes and monitoring its progression. We aimed to assess the interference posed by common Hb variants on popular HbA1c measurement systems. Methods A total of 63 variant and nonvariant samples with target values assigned by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC)-calibrated methods were included. We assessed 6 methods for measuring HbA1c in the presence of HbS, HbC, HbD, HbE, and fetal hemoglobin (HbF): 2 cation-exchange high-performance liquid chromatography (HPLC) methods (Bio-Rad D-100 and HLC-723 G8), a capillary electrophoresis (CE) method (Sebia Capillarys 3 TERA), an immunoassay (Roche c501), an enzyme assay system (Mindray BS-600M), and a boronate affinity method (Primus Premier Hb9210). Results The HbA1c results for nonvariant samples from the 6 methods were in good agreement with the IFCC-calibrated method results. The Bio-Rad D-100, Capillarys 3, Mindray BS-600M, Premier Hb9210, and Roche c501 showed no interference from HbS, HbC, HbD, and HbE. Clinically significant interference was observed for the HLC-723 G8 standard mode. Elevated HbF levels caused significant negative biases for all 6 methods, which increased with increasing HbF concentration. Conclusion Elevated levels of HbF can severely affect HbA1c measurements by borate affinity, immunoassays, and enzyme assays.
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