坐骨神经
轴突
医学
神经损伤
轴突切开术
神经营养因子
解剖
病理
线粒体
细胞生物学
内分泌学
内科学
麻醉
生物
中枢神经系统
受体
作者
Chi-Chung Kuo,Hong‐Lin Su,Tzu-Lin Chang,Chien-Yi Chiang,Meei-Ling Sheu,Fu‐Chou Cheng,Chun‐Jung Chen,Jason P. Sheehan,Hung-Chuan Pan
出处
期刊:Neurosurgery
[Oxford University Press]
日期:2017-02-20
卷期号:80 (3): 475-488
被引量:26
标识
DOI:10.1093/neuros/nyw090
摘要
Axon degeneration leads to cytoskeletal disassembly, metabolism imbalance, and mitochondrial dysfunction during neurodegeneration or nerve injury.In this study, we assess the possibility of mitigating axon degeneration by local injection of mitochondria in a crushed sciatic nerve.Sciatic nerve explants cocultured with mitochondria were assessed for the optimal dosage in local injection and nerve regeneration potential. The left sciatic nerve was crushed in Sprague-Dawley rats and then local injection of mitochondria into the distal end of the injured nerve was conducted for further assessment.Mitochondrial coculture attenuated cytoskeletal loss and oxidative stress in isolated nerve explants. In Vivo analyses also showed that mitochondrial transplantation improved animal neurobehaviors, electrophysiology of nerve conduction, and muscle activities. Mitochondria injection significantly attenuated the oxidative stress and increased the expression of neurotrophic factors both in injured nerves and denervated muscles, as well as restored muscular integrity, and increased the pool of muscular progenitor cells and total muscle weight.Mitochondria injection can protect injured nerves from axonal degeneration both in Vitro and in Vivo. This improvement was accompanied with the expression of neurotrophic factors as well as the reduction of oxidative stress, which may account for the functional recovery of both injured nerves and denervated muscles.
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