Characterization and immobilization of protease and lipase on chitin-starch material as a novel matrix

Protease and lipase were purified from sunflower seeds by frequent purification steps with molecular weights of 72.90 kDa and 27.50 kDa, respectively. The purified lipase and protease were immobilized on various carriers by different methods of immobilization including physical adsorption, ionic binding and covalent binding. The enzymes prepared by covalent binding on a new support materials were made via the combination of chitin and starch had the highest activates. The immobilization was carried out in a simpler way compared with the other immobilization methods which require various chemicals and complicated procedures which is difficult and expensive. The influence of reusability, pH, thermal and storage stability of immobilizing enzymes compared to the free enzyme were studied. The immobilizing protease and lipase with chitin and chitin + starch were used to catalyze reactions through enhanced washing process. After adding immobilizing enzymes with chitin and chitin starch, the stain removal percentage of detergents was enhanced by 78% and 84%, respectively. We approve that these novel immobilizing protease and lipase with chitin + starch present a new approach to the synthesis of multi enzyme biocatalysts from cheap and friendly environmental sources.