关节炎
类风湿性关节炎
胶原性关节炎
医学
基因剔除小鼠
免疫学
动物模型
实验病理学
生物
内科学
体内
受体
生物技术
作者
Lisette Bevaart,Margriet J. Vervoordeldonk,Paul P. Tak
出处
期刊:Methods in molecular biology
日期:2009-11-06
卷期号:: 181-192
被引量:57
标识
DOI:10.1007/978-1-60761-058-8_11
摘要
Collagen-induced arthritis (CIA) in mice is an animal model for rheumatoid arthritis (RA) and can be induced in DBA/1 and C57BL/6 mice using different protocols. The CIA model can be used to unravel mechanisms involved in the development of arthritis and is frequently used to study the effect of new therapeutics. The development of a CIA model in C57BL/6 mice recently enabled researchers to use knockout mice on this background for arthritis research. In this chapter, the protocol for induction of arthritis in both mice strains is described, including the monitoring of clinical arthritis and paw swelling in the mice during the experiment. Furthermore, protocols for decalcification of paws and for the detection of collagen-specific antibodies in mice sera are described.
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