多路复用
有孔小珠
流式细胞术
免疫分析
分析物
色谱法
化学
计算机科学
纳米技术
分子生物学
材料科学
抗体
生物
生物信息学
免疫学
复合材料
作者
R Varró,Roy Chen,Homero Sepulveda,John Apgar
标识
DOI:10.1007/978-1-59745-323-3_9
摘要
Analytical cytometry has significant potential beyond cellular analysis. The inherent capability of flow cytometers to efficiently discriminate between uniformly sized particles based on their intrinsic properties provides the foundation for multiplex bead assays. The technology can be exploited in designing immunoassays, Western blot-like antibody assays, and nucleic acid hybridization assays. This chapter focuses on immunoassay applications. The multiplex bead assays have recently evolved as a new and increasingly popular area for flow cytometry, becoming a good alternative to enzyme-linked immunosorbent assay for efficient evaluation of panels of analytes. This chapter provides detailed information about two bead platforms, the BD™ Cytometric Bead Array kits and the BD Cytometric Bead Array Flex Set Assays.
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