染色质
嘉雅宠物
染色体构象捕获
增强子
二价染色质
支架/基质附着区域
计算生物学
生物
染色质重塑
遗传学
细胞生物学
转录因子
基因
作者
Binbin Lai,Qingsong Tang,Wenfei Jin,Gangqing Hu,Darawalee Wangsa,Kairong Cui,Benjamin Z. Stanton,Gang Ren,Yi Ding,Ming Zhao,Shuai Liu,Jiuzhou Song,Thomas Ried,Keji Zhao
出处
期刊:Nature Methods
[Springer Nature]
日期:2018-08-27
卷期号:15 (9): 741-747
被引量:70
标识
DOI:10.1038/s41592-018-0107-y
摘要
Long-range chromatin interactions play critical roles in genome organization and regulation of transcription. We now report transposase-mediated analysis of chromatin looping (Trac-looping) for simultaneous detection of multiscale genome-wide chromatin interactions among regulatory elements and chromatin accessibility. With this technique, a bivalent oligonucleotide linker is inserted between two interacting regions such that the chromatin interactions are captured without prior chromatin fragmentation and proximity-based ligation. Application of Trac-looping to human CD4+ T cells revealed substantial reorganization of enhancer–promoter interactions associated with changes in gene expression after T cell receptor stimulation. Insertion of a bivalent linker between two regions of interest allows chromatin contacts to be probed without proximity ligation.
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