Lithocarpus polystachyus, also known as the sweet tea, is a plant of the family Fagaceae. It is widely distributed in southern China, India, and Thailand. The chloroplast (cp) genome of L. polystachyus is 161,217 bp in size containing 122 unique genes, including eight rRNA genes, 37 tRNA genes, and 77 protein-coding genes (PCGs). Phylogenetic analysis exhibited that L. polystachyus was most related to L. balansae.