次氯酸盐
氧化应激
次氯酸
线粒体
荧光
体内
化学
斑马鱼
氧化磷酸化
食腐动物
生物物理学
生物化学
生物
激进的
量子力学
基因
物理
生物技术
有机化学
作者
Huawei Niu,Keke Chen,Jiahui Xu,Xiaofei Zhu,Wenbo Cao,Zhenji Wang,Yong Ye,Yufen Zhao
标识
DOI:10.1016/j.snb.2019.126938
摘要
The excessive accumulation of hypochlorite could cause tissue damage and a range of diseases. However, the exact role of hypochlorite in biological activity remains elusive. In many of the reported probes for hypochlorite, DMSO which is an efficient HOCl and •OH scavenger was used as solvent. Besides, no fluorescent probes have been reported to quantify the accumulation of HOCl in living cells and in vivo. In order to reach this purpose, in this study, we report two mitochondria-targeted probes (L1 and L2) for detecting ClO−. These probes which were easy to be synthesized, shown high sensitivity and fast response (within 3 min) to ClO−. Fluorescent imaging of probes in MCF-7 cells and in zebrafish indicated that L1/L2 could be used for detecting ClO− in living cells and organisms. ClO− could also be used as a dual input logic gate for L1 and L2. Probe L2 could be used to monitor oxidative stress-related biological processes in nude mice.
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