脂肪酶
石墨烯
化学
催化作用
活动站点
纳米材料
分子动力学
纳米技术
化学工程
材料科学
组合化学
有机化学
酶
计算化学
工程类
作者
Motilal Mathesh,Binquan Luan,Taiwo O. Akanbi,Jeffrey K. Weber,Jingquan Liu,Colin J. Barrow,Ruhong Zhou,Wenrong Yang
出处
期刊:ACS Catalysis
[American Chemical Society]
日期:2016-06-09
卷期号:6 (7): 4760-4768
被引量:159
标识
DOI:10.1021/acscatal.6b00942
摘要
Lipases, which can be immobilized and reused for many reaction cycles, are important enzymes with many industrial applications. A key challenge in lipase immobilization for catalysis is to open the lipase lid and maintain it in an open conformation in order to expose its active site. Here we have designed "tailor-made" graphene-based nanosupports for effective lipase (QLM) immobilization through molecular engineering, which is in general a grand challenge to control biophysicochemical interactions at the nano–bio interface. It was observed that increasing hydrophobic surface increased lipase activity due to opening of the helical lid present on lipase. The molecular mechanism of lid opening revealed in molecular dynamics simulations highlights the role of hydrophobic interactions at the interface. We demonstrated that the open and active form of lipase can be achieved and tuned with an optimized activity through chemical reduction of graphene oxide. This research is a major step toward designing nanomaterials as a platform for enhancing enzyme immobilization/activity.
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