Preparation and characterization of oligopeptide modified PLGA-[ASP-PEG]biomimetic scaffolds

Objective To prepare a new biomimetic bone matrix material for tissue engineering by integrating polypeptide,which has excellent osteoinduction,with modified PLGA scaffold using cross- linkers.Methods The BMP-2-derived peptide was devised and synthetized,and PLGA-[ASP-PEG] was modified with polypeptide by methods of physical entrapment and chemical coupling,using 1-ethyl-3-(3- dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as cross-linkers.Under scanning electron microscopy (SEM),X-ray photoelectron spectroscopy (XPS) and by ion binding exper- iment,the products were identified.Results The contents of sulphur element on the surfaces of coupled composition and entrapped composition were 1.5% and 0.09% respectively.The amounts of ions attach- ing to the coupled composition were 0.126 mg at 12 h and 0.231 mg at 24 h,while amounts of ions at- taching to the entrapped composition were 0.053 mg at 12 h and 0.102 mg at 24 h respectively.The coupled composition showed greater ability to attach to ions than that of the entrapped composition.Con- clusion The coupled material established the basement of osteoinductive property and biomimetic miner- alized ability for the bone tissue engineering.