Characterization of the Protein Components of Matrix Stones Sheds Light on S100-A8 and S100-A9 Relevance in the Inflammatory Pathogenesis of These Rare Renal Calculi

No AccessJournal of UrologyInvestigative Urology1 Sep 2016Characterization of the Protein Components of Matrix Stones Sheds Light on S100-A8 and S100-A9 Relevance in the Inflammatory Pathogenesis of These Rare Renal Calculi Claudia Martelli, Valeria Marzano, Federica Iavarone, Liling Huang, Federica Vincenzoni, Claudia Desiderio, Irene Messana, Paolo Beltrami, Filiberto Zattoni, Pietro Manuel Ferraro, Noor Buchholz, Giorgia Locci, Gavino Faa, Massimo Castagnola, and Giovanni Gambaro Claudia MartelliClaudia Martelli Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Equal study contribution. More articles by this author , Valeria MarzanoValeria Marzano Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Equal study contribution. More articles by this author , Federica IavaroneFederica Iavarone Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Equal study contribution. More articles by this author , Liling HuangLiling Huang Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy More articles by this author , Federica VincenzoniFederica Vincenzoni Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy More articles by this author , Claudia DesiderioClaudia Desiderio Institute of Chemistry of Molecular Recognition, Italian National Research Council, Rome, Italy More articles by this author , Irene MessanaIrene Messana Department of Life and Environmental Sciences, Cagliari University, Cagliari, Italy More articles by this author , Paolo BeltramiPaolo Beltrami Urology Clinic, Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy More articles by this author , Filiberto ZattoniFiliberto Zattoni Urology Clinic, Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy More articles by this author , Pietro Manuel FerraroPietro Manuel Ferraro Nephrology Division, Institute of Internal Medicine, Catholic University, Rome, Italy More articles by this author , Noor BuchholzNoor Buchholz U-merge (Urology in Emerging Countries), London, United Kingdom More articles by this author , Giorgia LocciGiorgia Locci Pathology Division, Department of Surgery, Cagliari University, Cagliari, Italy More articles by this author , Gavino FaaGavino Faa Pathology Division, Department of Surgery, Cagliari University, Cagliari, Italy More articles by this author , Massimo CastagnolaMassimo Castagnola Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Institute of Chemistry of Molecular Recognition, Italian National Research Council, Rome, Italy More articles by this author , and Giovanni GambaroGiovanni Gambaro Nephrology Division, Institute of Internal Medicine, Catholic University, Rome, Italy More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2016.04.064AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Among the different types of kidney stones, matrix stones are uncommon urinary calculi composed of a soft, pliable, amorphous substance with little crystalline content. To gain insight into the pathogenesis we investigated the protein component by analyzing the proteomic profiles of surgically removed matrix stones. Materials and Methods: A total of 5 stones were harvested from 4 patients who underwent surgery for medical reasons at 3 clinical centers during a 7-year period. Matrix stone proteome characterization was performed by mass spectrometry based techniques using an integrated top-down/bottom-up proteomic platform. Results: We identified 142 nonredundant proteins and peptides across all samples. Neutrophil defensin 1, and proteins S100-A8 and S100-A9 were the main components of these renal calculi. Conclusions: The abundance of identified inflammatory molecules points to an inflammatory process as the event that initializes soft calculi formation rather than as a consequence of such formation. The post-translational oxidative changes in S100-A8 and A9, and the presence of thymosin β-4, granulins and ubiquitin also suggest the intervention of host defenses through a superimposed, vigorous counter inflammatory process. The post-translational changes seen in the proteins and peptides, and the known self-assembling capability of S100-A8 and S100-A9 probably explain the gelatinous consistency of these stones. References 1 : V. Fibrinous calculi in the kidney. Ann Surg1908; 48: 378. Google Scholar 2 : The management of renal matrix calculi: a single-centre experience over 5 years. BJU Int2009; 103: 810. Google Scholar 3 : The endourological treatment of renal matrix stones. Urol Int2014; 93: 394. Google Scholar 4 : Urinary matrix calculi consisting of microfibrillar protein in patients on maintenance hemodialysis. Kidney Int1979; 16: 722. 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Google Scholar © 2016 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 196Issue 3September 2016Page: 911-918Supplementary Materials Advertisement Copyright & Permissions© 2016 by American Urological Association Education and Research, Inc.Keywordsproteomicskidney calculiinflammationMetricsAuthor Information Claudia Martelli Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Equal study contribution. More articles by this author Valeria Marzano Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Equal study contribution. More articles by this author Federica Iavarone Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Equal study contribution. More articles by this author Liling Huang Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy More articles by this author Federica Vincenzoni Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy More articles by this author Claudia Desiderio Institute of Chemistry of Molecular Recognition, Italian National Research Council, Rome, Italy More articles by this author Irene Messana Department of Life and Environmental Sciences, Cagliari University, Cagliari, Italy More articles by this author Paolo Beltrami Urology Clinic, Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy More articles by this author Filiberto Zattoni Urology Clinic, Department of Surgical, Oncological and Gastroenterological Sciences, University of Padova, Padova, Italy More articles by this author Pietro Manuel Ferraro Nephrology Division, Institute of Internal Medicine, Catholic University, Rome, Italy More articles by this author Noor Buchholz U-merge (Urology in Emerging Countries), London, United Kingdom More articles by this author Giorgia Locci Pathology Division, Department of Surgery, Cagliari University, Cagliari, Italy More articles by this author Gavino Faa Pathology Division, Department of Surgery, Cagliari University, Cagliari, Italy More articles by this author Massimo Castagnola Institute of Biochemistry and Clinical Biochemistry, Catholic University, Rome, Italy Institute of Chemistry of Molecular Recognition, Italian National Research Council, Rome, Italy More articles by this author Giovanni Gambaro Nephrology Division, Institute of Internal Medicine, Catholic University, Rome, Italy More articles by this author Expand All Advertisement PDF downloadLoading ...