Establishment of HPLC fingerprint and its application in identification of Folium Apocyni Veneti

Objective To establish chromatographic fingerprint of Folium Apocyni Veneti by HPLC and identify Folium Apocyni Veneti from its adulterants.Methods HPLC Gradient elution was applied to establish the chromatographic fingerprint andComputer-Aided Similarity Evaluation Systemwas used in data analyses.Results This chromatographic fingerprint method has good precision,stability,and re- peatability.Twelve common peaks were marked.There are notable differences in chromatographic finger- prints between Folium Apocyni Veneti and its adulterants.Conclusion This chromatographic fingerprint method can be used to evaluate the quality of Folium Apocyni Veneti.