Evaluation of the Orbitrap Mass Spectrometer for the Molecular Fingerprinting Analysis of Natural Dissolved Organic Matter

轨道轨道 化学 傅里叶变换离子回旋共振 质谱法 溶解有机碳 质谱 分析化学(期刊) 环境化学 色谱法
作者
Jeffrey A. Hawkes,Thorsten Dittmar,Carlo Patriarca,Lars J. Tranvik,Jonas Bergquist
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:88 (15): 7698-7704 被引量:120
标识
DOI:10.1021/acs.analchem.6b01624
摘要

We investigated the application of the LTQ-Orbitrap mass spectrometer (LTQ-Velos Pro, Thermo Fisher) for resolving complex mixtures of natural aquatic dissolved organic matter (DOM) and compared this technique to the more established state-of-the-art technique, Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS, Bruker Daltonics), in terms of the distribution of molecular masses detected and the reproducibility of the results collected. The Orbitrap was capable of excellent reproducibility: Bray-Curtis dissimilarity between duplicate measurements was 2.85 ± 0.42% (mean ± standard deviation). The Orbitrap was also capable of the detection of most major ionizable organic molecules in typical aquatic mixtures, with the exception of most sulfur and phosphorus containing masses. This result signifies that the Orbitrap is an appropriate technique for the investigation of very subtle biogeochemical processing of bulk DOM. The lower costs (purchase and maintenance) and wider availability of Orbitrap mass spectrometers in university departments means that the tools necessary for research into DOM processing at the molecular level should be accessible to a much wider group of scientists than before. The main disadvantage of the technique is that substantially fewer molecular formulas can be resolved from a complex mixture (roughly one third as many), meaning some loss of information. In balance, most biogeochemical studies that aim at molecularly fingerprinting the source of natural DOM could be satisfactorily carried out with Orbitrap mass spectrometry. For more targeted metabolomic studies where individual compounds are traced through natural systems, FTICR-MS remains advantageous.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
hutian完成签到,获得积分10
刚刚
刚刚
1秒前
默默荔枝完成签到 ,获得积分10
1秒前
SYLH应助打工人采纳,获得10
1秒前
王w完成签到,获得积分10
1秒前
SYLH应助wen采纳,获得10
2秒前
bkagyin应助夏梦园采纳,获得10
2秒前
如如要动发布了新的文献求助200
2秒前
3秒前
FFFFFFG完成签到,获得积分10
4秒前
4秒前
5秒前
6秒前
sigla发布了新的文献求助10
6秒前
wss完成签到,获得积分10
6秒前
跳跃忆灵完成签到,获得积分10
6秒前
左凝阳发布了新的文献求助10
6秒前
风中幻梦发布了新的文献求助10
7秒前
7秒前
7秒前
SYLH应助飞云采纳,获得10
7秒前
研友_VZG7GZ应助疗效采纳,获得10
8秒前
8秒前
8秒前
一朵梅花完成签到,获得积分10
10秒前
11秒前
monoklatt发布了新的文献求助30
11秒前
wss发布了新的文献求助10
12秒前
脑洞疼应助大剑日太刀采纳,获得10
12秒前
Synan完成签到,获得积分10
13秒前
李健的小迷弟应助orange9采纳,获得10
13秒前
tx完成签到,获得积分20
14秒前
艾小晗发布了新的文献求助20
14秒前
asbefore完成签到,获得积分10
14秒前
lzc完成签到,获得积分10
14秒前
寒冷兔子发布了新的文献求助10
14秒前
Imwang完成签到,获得积分10
15秒前
15秒前
蓝天白云完成签到,获得积分10
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Toward a Combinatorial Approach for the Prediction of IgG Half-Life and Clearance 500
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3970120
求助须知:如何正确求助?哪些是违规求助? 3514810
关于积分的说明 11176124
捐赠科研通 3250136
什么是DOI,文献DOI怎么找? 1795198
邀请新用户注册赠送积分活动 875647
科研通“疑难数据库(出版商)”最低求助积分说明 804964