Protective immunity conferred by porcine circovirus 2 ORF2-based DNA vaccine in mice

猪圆环病毒 免疫原性 dna疫苗 圆环病毒 病毒学 生物 免疫 接种疫苗 重组DNA 抗体 免疫系统 免疫学 免疫 病毒 基因 生物化学
作者
S Sylla,Yanlong Cong,Yixue Sun,Guilian Yang,Xuemei Ding,Zhan‐Qing Yang,Yulong Zhou,Minnan Yang,Chunfeng Wang,Zhuang Ding
出处
期刊:Microbiology and Immunology [Wiley]
卷期号:58 (7): 398-408 被引量:13
标识
DOI:10.1111/1348-0421.12158
摘要

Post-weaning multisystemic wasting syndrome (PMWS) associated with porcine circovirus type 2 (PCV2) has caused the swine industry significant health challenges and economic damage. Although inactivated and subunit vaccines against PMWS have been used widely, so far no DNA vaccine is available. In this study, with the aim of exploring a new route for developing a vaccine against PCV2, the immunogenicity of a DNA vaccine was evaluated in mice. The pEGFP-N1 vector was used to construct a PCV2 Cap gene recombinant vaccine. To assess the immunogenicity of pEGFP-Cap, 80 BALB/c mice were immunized three times at 2 weekly intervals with pEGFP-Cap, LG-strain vaccine, pEGFP-N1 vector or PBS and then challenged with PCV2. IgG and cytokines were assessed by indirect ELISA and ELISA, respectively. Specimens stained with hematoxylin and eosin (HE) and immunohistochemistry (IHC) techniques were examined histopathologically. It was found that vaccination of the mice with the pEGFP-Cap induced solid protection against PCV2 infection through induction of highly specific serum IgG antibodies and cytokines (IFN-γ and IL-10), and a small PCV2 viral load. The mice treated with the pEGFP-Cap and LG-strain developed no histopathologically detectable lesions (HE stain) and IHC techniques revealed only a few positive cells. Thus, this study demonstrated that recombinant pEGFP-Cap substantially alleviates PCV2 infection in mice and provides evidence that a DNA vaccine could be an alternative to PCV2 vaccines against PMWS.
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