Cell adhesion biomaterial based on mussel adhesive protein fused with RGD peptide

细胞粘附 粘附 纤维连接蛋白 生物粘附 融合蛋白 生物材料 细胞 材料科学 重组DNA 生物化学 化学 细胞生物学 分子生物学 生物 纳米技术 复合材料 基因 药物输送
作者
Dong Soo Hwang,Sung Bo Sim,Hyung Joon
出处
期刊:Biomaterials [Elsevier BV]
卷期号:28 (28): 4039-4046 被引量:194
标识
DOI:10.1016/j.biomaterials.2007.05.028
摘要

Previously, we designed and constructed a hybrid of the mussel adhesive protein (MAP) fp-151, which is a fusion protein with six type 1 (fp-1) decapeptide repeats at each type 5 (fp-5) terminus. Through various cell-adhesion analyses, we previously demonstrated that fp-151 has the potential to be used as a cell or tissue bioadhesive. In the present study, to improve the cell-adhesion properties of fp-151, we designed a new cell-adhesive protein, fp-151-RGD, which is a fusion with the GRGDSP residues, a RGD peptide sequence that has previously been identified at the cell-attachment site of fibronectin, at the C-terminus of fp-151. Although recombinant fp-151-RGD maintained the advantages associated with fp-151, such as a high production yield in Escherichia coli and simple purification, it showed superior spreading ability, which is important for cell proliferation under serum-free conditions, as well as better cell-adhesion ability compared with other commercially produced cell-adhesion materials such as poly-l-lysine (PLL) and the naturally extracted MAP mixture Cell-Tak. The excellent adhesion and spreading abilities of fp-151-RGD might be due to the fact that it utilizes three types of cell-binding mechanisms: DOPA adhesion of Cell-Tak, cationic binding force of PLL, and RGD sequence-mediated adhesion of fibronectin. Therefore, the new recombinant fp-151-RGD is suitable for use as a cell-adhesion material in cell culture or tissue engineering, and in any other area where efficient cell adhesion is required.
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