Construct Functional Oligonucleotides Simultaneous Targeting FEN1 and MIR-21 Integrate Photothermal Therapy to Inhibit Cancer Cell Growth

Abundant investigators have devoted their efforts to proceed the development of novel strategies aimed at anti-tumor through regulating gene expression negatively. Flap structure-specific endonuclease 1 (FEN1) received widespread attention in becoming a potential target for breast cancer. In this study, a prominent protocol based on small molecular nucleic acids was reasonably demonstrated. Various oligonucleotides containing FEN1 cleavage site and anti-miRNA21 sequences were designed and synthesized as a kind of disturbance factor to FEN1 and miRNA21. In order to obtain an ideal therapeutic outcome, graphene oxide (GO) was also employed, which rendered the whole system with photothermal therapy (PTT) effect. Finally, a novel trinity treatment system came into being with the characteristic of combined therapy involving miRNA21 silencing, FEN1 inhibition and PTT. In short, it is our hope that the designed multifunctional system could provide a promising use for the development of anti-tumor therapy in the foreseeable future.