细胞生物学
抄写(语言学)
发起人
计算生物学
转录调控
RNA聚合酶Ⅱ
增强子
作者
Jorge Trojanowski,Lukas Frank,Anne Rademacher,Pranas Grigaitis,Karsten Rippe
标识
DOI:10.1101/2021.01.27.428421
摘要
Summary Transcription factors (TFs) consist of a DNA binding and an activation domain (AD) that are considered to be independent and exchangeable modules. However, recent studies conclude that also the physico-chemical properties of the AD can control TF assembly at chromatin by driving a phase separation into transcriptional condensates. Here, we dissected transcription activation by comparing different synthetic TFs at a reporter gene array with real-time single-cell fluorescence microscopy readouts. In these experiments, binding site occupancy, residence time and co-activator recruitment in relation to multivalent TF interactions were compared. While phase separation propensity and activation strength of the AD were correlated, the actual formation of liquid-like TF droplets had a neutral or inhibitory effect on transcription activation. Rather, we conclude that multivalent AD mediated interactions increase the transcription activation capacity of a TF by stabilizing chromatin binding and mediating the recruitment of co-activators independent of phase separation.
科研通智能强力驱动
Strongly Powered by AbleSci AI