核酸酶
清脆的
核酸
计算生物学
生物分析
脱氧核酶
滚动圆复制
回文
DNA
生物
纳米技术
聚合酶
遗传学
基因
材料科学
作者
Xiaolin Ge,Tian Meng,Xiao Tan,Yangdao Wei,Zhenzhen Tao,Zhiqing Yang,Fengge Song,Peng Wang,Yi Wan
标识
DOI:10.1016/j.bios.2021.113350
摘要
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-associated nuclease (Cas) based biosensing system provides a novel genomic diagnostic tool for pathogenic detection. However, most of the discovered Cas effectors have poor single strand DNA (ssDNA) target recognition capability with the constraint of protospacer adjacent motif (PAM) sites, which are not suitable for universal pathogenic diagnosis. Herein, we developed a highly sensitive and specific fluorescence tool for bacterial detection by utilizing the unique collateral cleavage activity of a Cas14a1-mediated nucleic acid detection platform (CMP). We combine CMP with molecular amplification to build a CRISPR-Cas based bioanalysis technique, offering fast nucleic acid detection with high sensitivity and specificity. This technique can identify different species of pathogens in milk samples with excellent accuracy. The CMP technique is a promising platform for pathogenic genomic diagnostic in biomedicine and food safety field.
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