化学
胶体金
检出限
色谱法
荧光
试剂
纳米颗粒
福尔马赞
比色法
核化学
线性范围
肉眼
分析物
作者
Ningning Yue,Dongmei Li,Aiping Fan
出处
期刊:Analytical Sciences
[Japan Society for Analytical Chemistry]
日期:2021-05-14
卷期号:37 (11): 1583-1587
被引量:5
标识
DOI:10.2116/analsci.21p115
摘要
The development of simple and sensitive detection methods for tetracyclines (TCs) is crucial for their routine detection. The present study developed a colorimetric method for the detection of TCs based on the in-situ generation of AuNPs, which were subsequently coupled with a gold staining reaction. Briefly, TCs containing phenolic groups reduce HAuCl4 to form gold nanoparticles (AuNPs) as gold seeds. In the gold staining process, the gold seeds catalyze the reduction of HAuCl4 by NH2OH to form gold atoms that deposit on the surface of AuNPs, resulting in the enlargement of AuNPs. Sensitive detection of TCs was achieved by employing the gold staining technique. As low as 14, 18.9, and 1.98 nM of oxytetracycline (OTC), tetracycline (TC), and doxycycline (DC), respectively, could be sensitively detected. The proposed method also exhibited good repeatability and specificity, and then was applied to the determination of OTC in milk samples.
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