Hsa_circ_0014879 regulates the radiosensitivity of esophageal squamous cell carcinoma through miR-519-3p/CDC25A axis

辐射敏感性 CDC25A型 抗辐射性 癌症研究 细胞生长 细胞周期 小RNA 下调和上调 生物 化学 免疫印迹 细胞 基因沉默 细胞培养 分子生物学 医学 细胞周期检查点 内科学 放射治疗 基因 生物化学 遗传学
作者
Zihao Liu,Xiyan Lu,Linchun Wen,Chuanwen You,Xiaowei Jin,Jingying Liu
出处
期刊:Anti-Cancer Drugs [Lippincott Williams & Wilkins]
卷期号:33 (1): e349-e361 被引量:14
标识
DOI:10.1097/cad.0000000000001213
摘要

Circular RNAs (circRNAs) play critical roles in regulating the radiosensitivity of various cancers, including esophageal squamous cell carcinoma (ESCC). This research aimed to explore the role and potential mechanism of hsa_circ_0014879 in regulating ESCC radioresistance. The levels of hsa_circ_0014879, microRNA-519-3p (miR-519-3p) and cell division cycle 25A (CDC25A) were measured using quantitative real-time PCR or western blot. Cell proliferation was evaluated by colony formation assay. Cell migration and invasion were assessed by transwell and scratch assays. The levels of epithelial-mesenchymal transition (EMT)-related proteins were detected by western blot. Xenograft assay was used to analyze the effect of hsa_circ_0014879 on radiosensitivity in vivo. The binding relationship among hsa_circ_0014879, miR-519-3p and CDC25A was confirmed by dual-luciferase reporter assay. Hsa_circ_0014879 and CDC25A were upregulated, whereas miR-519-3p was downregulated in radio-resistant ESCC tissues and cells. Depletion of hsa_circ_0014879 suppressed the proliferation, migration and invasion of radio-resistant ESCC cells. Hsa_circ_0014879 knockdown elevated radiosensitivity of radio-resistant cells by modulating miR-519-3p. Moreover, miR-519-3p enhanced the radiosensitivity of radio-resistant cells by targeting CDC25A. Also, hsa_circ_0014879 upregulated CDC25A via sponging miR-519-3p. Hsa_circ_0014879 silencing enhanced the radiosensitivity of ESCC via regulating the miR-519-3p/CDC25A pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
邓佳鑫Alan应助TuTuesday采纳,获得10
刚刚
刚刚
青竹完成签到,获得积分10
1秒前
YiXianCoA发布了新的文献求助10
1秒前
小鱼完成签到,获得积分10
1秒前
2秒前
量子星尘发布了新的文献求助10
2秒前
gs发布了新的文献求助10
2秒前
yi发布了新的文献求助10
3秒前
无花果应助凡夕木叶采纳,获得10
3秒前
3秒前
娴娴超爱笑完成签到,获得积分10
3秒前
我是老大应助wangs采纳,获得10
4秒前
4秒前
olivia完成签到,获得积分10
5秒前
5秒前
5秒前
没有昵称发布了新的文献求助20
6秒前
咿呀发布了新的文献求助10
6秒前
6秒前
7秒前
CipherSage应助纪尔蓝采纳,获得10
7秒前
思源应助罗大壮采纳,获得10
7秒前
浮生发布了新的文献求助30
7秒前
艾斯完成签到,获得积分10
8秒前
zxy完成签到,获得积分10
8秒前
8秒前
开心寄松发布了新的文献求助10
9秒前
9秒前
果果发布了新的文献求助10
9秒前
9秒前
锂安完成签到,获得积分10
10秒前
yu完成签到,获得积分10
10秒前
hang发布了新的文献求助30
10秒前
anna1992发布了新的文献求助10
11秒前
577610822发布了新的文献求助30
11秒前
mendy_ng完成签到,获得积分10
11秒前
12秒前
13秒前
13秒前
高分求助中
Ophthalmic Equipment Market by Devices(surgical: vitreorentinal,IOLs,OVDs,contact lens,RGP lens,backflush,diagnostic&monitoring:OCT,actorefractor,keratometer,tonometer,ophthalmoscpe,OVD), End User,Buying Criteria-Global Forecast to2029 2000
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Cognitive Neuroscience: The Biology of the Mind (Sixth Edition) 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 588
Christian Women in Chinese Society: The Anglican Story 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3961655
求助须知:如何正确求助?哪些是违规求助? 3507998
关于积分的说明 11139004
捐赠科研通 3240407
什么是DOI,文献DOI怎么找? 1790947
邀请新用户注册赠送积分活动 872683
科研通“疑难数据库(出版商)”最低求助积分说明 803306