辐射敏感性
CDC25A型
抗辐射性
癌症研究
细胞生长
细胞周期
小RNA
下调和上调
生物
化学
免疫印迹
细胞
基因沉默
细胞培养
分子生物学
医学
细胞周期检查点
内科学
放射治疗
基因
生物化学
遗传学
作者
Zihao Liu,Xiyan Lu,Linchun Wen,Chuanwen You,Xiaowei Jin,Jingying Liu
出处
期刊:Anti-Cancer Drugs
[Lippincott Williams & Wilkins]
日期:2021-08-16
卷期号:33 (1): e349-e361
被引量:14
标识
DOI:10.1097/cad.0000000000001213
摘要
Circular RNAs (circRNAs) play critical roles in regulating the radiosensitivity of various cancers, including esophageal squamous cell carcinoma (ESCC). This research aimed to explore the role and potential mechanism of hsa_circ_0014879 in regulating ESCC radioresistance. The levels of hsa_circ_0014879, microRNA-519-3p (miR-519-3p) and cell division cycle 25A (CDC25A) were measured using quantitative real-time PCR or western blot. Cell proliferation was evaluated by colony formation assay. Cell migration and invasion were assessed by transwell and scratch assays. The levels of epithelial-mesenchymal transition (EMT)-related proteins were detected by western blot. Xenograft assay was used to analyze the effect of hsa_circ_0014879 on radiosensitivity in vivo. The binding relationship among hsa_circ_0014879, miR-519-3p and CDC25A was confirmed by dual-luciferase reporter assay. Hsa_circ_0014879 and CDC25A were upregulated, whereas miR-519-3p was downregulated in radio-resistant ESCC tissues and cells. Depletion of hsa_circ_0014879 suppressed the proliferation, migration and invasion of radio-resistant ESCC cells. Hsa_circ_0014879 knockdown elevated radiosensitivity of radio-resistant cells by modulating miR-519-3p. Moreover, miR-519-3p enhanced the radiosensitivity of radio-resistant cells by targeting CDC25A. Also, hsa_circ_0014879 upregulated CDC25A via sponging miR-519-3p. Hsa_circ_0014879 silencing enhanced the radiosensitivity of ESCC via regulating the miR-519-3p/CDC25A pathway.
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