Screening Technique Based on Seed and Early Seedling Parameters for Cold Tolerance of Selected F2-Derived F3 Rice Genotypes under Controlled Conditions

胚芽鞘 胚根 发芽 苗木 生物 人口 耐寒性 园艺 数量性状位点 农学 植物 医学 遗传学 环境卫生 基因
作者
Javeed A. Lone,Asif B. Shikari,Najeeb ul Rehman Sofi,Sajad A Ganie,Manmohan Sharma,Mamta Sharma,Mukul Kumar,Muhammad Hamzah Saleem,Khalid S. Almaary,Mohamed S. Elshikh,Yheni Dwiningsih,Muhammad Ammar Raza
出处
期刊:Sustainability [MDPI AG]
卷期号:14 (14): 8447-8447 被引量:10
标识
DOI:10.3390/su14148447
摘要

The cold tolerance studies were carried out in a bi-parental F2 population of a cross between tolerant and susceptible parents (SKUA-529 and HEERA, respectively). The purpose was to screen the individuals of a population for primary cold-tolerance-related attributes. The information generated has a direct application and use in identifying cold tolerance quantitative trait loci (QTLs) and further can be used for genotyping with an appropriate marker system. The screening was carried out on F2-derived F3 seeds and F3 plants for seedling and agronomic traits, respectively. Two tests measuring cold tolerance were conducted. In experiment I, seeds were germinated for 28 days at 13 °C and 7 days at 28 °C, and in experiment II, the seeds were germinated for 72 h at 28 °C, 96 h at 13 °C, and once more for 72 h at 28 °C. Coleoptile length, germination percentage, and radical reduction percentages were all measured in experiment I. The radicle and coleoptile regeneration in experiment II were measured after the cold period. The improvement in cold tolerance was achieved through radicle regrowth, as evidenced by the difference between the second and first measurements. The individual lines from the F2:3 population that recorded high germination (%) were #21, #13, #14, and #15. The percentage of coleoptile length (PERCOL %) was observed to be between the ranges of 23.33% to 53.00%. The reduction in coleoptile length (REDCOL %) was also obtained, and there was less reduction in #15, #16, and #14 and it had a range between 38.46% and 75%. Radicle regrowth (REDRAG) was high at 13 °C in #7, #11, #30, #35, and #36. Survival of the seedling range was between 33.33% and up to 100%, and the highest survival rate was observed in #16. The main objective of this rotation in temperature was to emulate field conditions where there has been a drop in temperature. The evaluations were done for primary cold stress tolerance traits, and it was found that most of these traits exhibited high variability. The mapping population developed may be utilized to generate a linkage map and locate QTLs for tolerance to cold stress in rice. Further, the identified donors for cold tolerance may be utilized for breeding programs aimed at the transfer of low-temperature stress tolerance into susceptible backgrounds. In general, a genotype with improved seedling germination rates, growth rates, and leaf yellowing scores; high seedling survival; lesser reduction in coleoptile length and in radicle development; and recovery following a cold shock at the seedling stage demonstrated its cold resistance. Genotypes with a low germination percentage, a greater number of days to germination, slow growth rate and higher leaf yellowing score, high reduction in coleoptile and radicle growth, and reduced seedling survival indicated cold susceptibility.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
康桥发布了新的文献求助10
1秒前
幽壑之潜蛟应助枫叶采纳,获得10
1秒前
orixero应助枫叶采纳,获得10
1秒前
1秒前
4秒前
ppp完成签到,获得积分10
4秒前
黄洁滢发布了新的文献求助10
5秒前
稳重采枫发布了新的文献求助10
5秒前
5秒前
怎么可能会凉完成签到 ,获得积分10
5秒前
万能图书馆应助小洛采纳,获得10
6秒前
7秒前
小汤发布了新的文献求助10
8秒前
epmoct完成签到 ,获得积分10
8秒前
snow_dragon完成签到 ,获得积分10
8秒前
卢佳伟发布了新的文献求助10
8秒前
Chen发布了新的文献求助10
9秒前
共享精神应助Starry采纳,获得10
10秒前
10秒前
斯文涔雨发布了新的文献求助10
10秒前
11秒前
Owen应助111采纳,获得10
12秒前
13秒前
共享精神应助老实大米采纳,获得10
13秒前
稳重采枫完成签到,获得积分10
14秒前
14秒前
adamchris发布了新的文献求助150
15秒前
15秒前
西江婉儿完成签到 ,获得积分10
16秒前
zhunzhunzhun发布了新的文献求助10
16秒前
心平气和完成签到,获得积分10
16秒前
李爱国应助Yuqing采纳,获得10
17秒前
18秒前
3w学术完成签到 ,获得积分10
19秒前
clyhg发布了新的文献求助10
19秒前
大模型应助爱听歌的青筠采纳,获得10
19秒前
彩色的云朵完成签到,获得积分10
20秒前
完美世界应助Yuan采纳,获得10
20秒前
epmoctzyw完成签到 ,获得积分10
20秒前
科研通AI5应助南苏采纳,获得10
21秒前
高分求助中
Continuum thermodynamics and material modelling 3000
Production Logging: Theoretical and Interpretive Elements 2500
Healthcare Finance: Modern Financial Analysis for Accelerating Biomedical Innovation 2000
Applications of Emerging Nanomaterials and Nanotechnology 1111
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Theory of Block Polymer Self-Assembly 750
지식생태학: 생태학, 죽은 지식을 깨우다 700
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3483701
求助须知:如何正确求助?哪些是违规求助? 3072962
关于积分的说明 9128742
捐赠科研通 2764574
什么是DOI,文献DOI怎么找? 1517253
邀请新用户注册赠送积分活动 701974
科研通“疑难数据库(出版商)”最低求助积分说明 700831