Method for loading liposomes with soybean protein isolate hydrolysate influences the antioxidant efficiency of liposomal systems: Adding after liposomes formation or before lipid film hydration

脂质体 化学 水解物 吸附 色谱法 水解 脂质双层 生物物理学 生物化学 有机化学 生物
作者
Chong Chen,Dongxiao Sun‐Waterhouse,Jie Zhao,Yi Zhang,Geoffrey I. N. Waterhouse,Lijing Lin,Mouming Zhao,Weizheng Sun
出处
期刊:Food Hydrocolloids [Elsevier BV]
卷期号:129: 107629-107629 被引量:13
标识
DOI:10.1016/j.foodhyd.2022.107629
摘要

This research investigated the method for loading liposomes with soybean protein isolate hydrolysate (SPIH) to create stable and antioxidative protein hydrolysate-containing liposomes. SPIH (SPIH-AF) prepared via a two-step hydrolysis by alcalase and flavourzyme was added at different concentrations to liposomes by two methods. More effective adsorption of SPIH-AF onto liposomal membrane occurred when it was added at 0.5 mg/mL and distributed in both the inner and outer water phases (addition before lipid film hydration), or added at lower concentrations (< 0.192 mg/mL) and distributed in the outer water phase (addition after liposomes formation). SPIH-AF concentration and distribution directly influenced its early rate of inhibition on lipid oxidation and membrane properties. SPIH-AF in the outer water phase functioned more effectively to inhibit lipid oxidation products accumulation. The interactions between SPIH-AF and liposomes altered SPIH-AF's secondary structures. Compared with Trp-liposomes interactions, Tyr-liposomes interactions were more sensitive to SPIH-AF concentration and distribution. SPIH-AF distribution affected strongly the electrostatic interactions (but weakly the hydrophobic interactions). Tyr/Trp-liposomes interactions were more powerful when SPIH-AF was located in both the inner and outer water phases at higher concentrations. • SPIH-AF distribution and concentration affected early lipid oxidation inhibition. • Tyr-liposome interactions were more sensitive to SPIH-AF distribution/concentration. • SPIH-AF distribution affected its electrostatic interactions with lipid membrane. • Tailored loading methods allow SPIH-AF effective adsorption onto liposomal membrane.
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