亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Apoptosis and blood-testis barrier disruption during male reproductive dysfunction induced by PAHs of different molecular weights

细胞凋亡 粘合连接 生殖毒性 支持细胞 氧化应激 分子生物学 化学 苯并(a)芘 微核试验 流式细胞术 男科 标记法 荧蒽 毒性 生物 精子发生 生物化学 内分泌学 细胞 致癌物 医学 环境化学 有机化学 钙粘蛋白
作者
Lin Zhang,Xiaoli Ji,Hang Fan,Xuan Wu,Ning Tang,Qing Wu
出处
期刊:Environmental Pollution [Elsevier BV]
卷期号:300: 118959-118959 被引量:24
标识
DOI:10.1016/j.envpol.2022.118959
摘要

The association between polycyclic aromatic hydrocarbons (PAHs) and male reproductive dysfunction has attracted increasing attention. The purpose of this study was to compare the male reproductive toxicity of multiple PAHs and to investigate the underlying molecular mechanisms. TM4 cells (mouse testicular Sertoli cells, SCs) were treated with benzo(a)pyrene (BaP), pyrene (Py), fluoranthene (Fl) and phenanthrene (Phe) (0, 0.1, 1, 10, 50, or 100 μM) for varying time points (4, 12, 24, or 48 h), and male C57BL/6 mice were administered BaP and Py (0, 10, 50, or 100 mg/kg body weight) for 14 days based on the cell experimental results. Histopathological examination, western blotting, ELISA, biochemical assays, RT-PCR, flow cytometry, JC-1 staining and trans-epithelium electrical resistance (TEER) measurements were used to assess apoptosis, blood-testis barrier (BTB) integrity, intracellular calcium ([Ca2+]i) concentrations and oxidative stress (OS). The results revealed that the mRNA levels and enzymatic activities of CYP450 and GST family members; levels of ROS, MDA, cleaved caspase 3 (c-caspase 3), caspase 9, Bax, and cytochrome C (CytC); and numbers of TUNEL-positive cells were significantly increased by BaP and Py, while levels of AhR, GSH, SOD, CAT, Bcl-2 and ΔΨm were decreased. Additionally, BaP and Py notably interfered with tight junctions (TJs) and adherens junctions (AJs) in the BTB. Intriguingly, BaP, but not Py, induced [Ca2+]i overload and gap junction (GJ) destruction. There was no dramatic effect of Fl and/or Phe on any of the above parameters except that slight cytotoxicity was observed with higher doses of Fl. Collectively, these findings showed that BaP and Py elicited SC apoptosis and BTB disruption involving mitochondrial dysfunction and OS, but [Ca2+]i fluctuation and GJ injury were only observed with BaP-induced reproductive toxicity. The male reproductive toxicity of the selected PAHs was ranked in the order of BaP > Py > Fl > Phe.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
叫滚滚发布了新的文献求助20
4秒前
vitamin完成签到 ,获得积分10
6秒前
情怀应助叫滚滚采纳,获得10
26秒前
33秒前
40秒前
叫滚滚发布了新的文献求助10
44秒前
1分钟前
1分钟前
gyh发布了新的文献求助10
1分钟前
Owen应助獭兔采纳,获得10
1分钟前
小学生的练习簿完成签到,获得积分0
1分钟前
kmzzy完成签到,获得积分10
1分钟前
1分钟前
獭兔发布了新的文献求助10
1分钟前
Jasper应助科研通管家采纳,获得10
1分钟前
Hello应助科研通管家采纳,获得10
1分钟前
獭兔完成签到,获得积分10
2分钟前
2分钟前
wqc2060完成签到,获得积分10
2分钟前
FIN发布了新的文献求助50
3分钟前
sailingluwl完成签到,获得积分10
3分钟前
3分钟前
共享精神应助科研通管家采纳,获得10
3分钟前
慕青应助科研通管家采纳,获得10
3分钟前
Ava应助科研通管家采纳,获得10
3分钟前
小余同学完成签到,获得积分10
4分钟前
小余同学发布了新的文献求助10
4分钟前
CipherSage应助吴彦祖采纳,获得10
4分钟前
糊涂的青烟完成签到 ,获得积分10
4分钟前
wangye完成签到 ,获得积分10
4分钟前
4分钟前
4分钟前
吴彦祖发布了新的文献求助10
5分钟前
lyj完成签到 ,获得积分10
5分钟前
5分钟前
脑洞疼应助生动之云采纳,获得10
5分钟前
bji完成签到,获得积分10
5分钟前
大模型应助bji采纳,获得10
5分钟前
5分钟前
SciGPT应助科研通管家采纳,获得10
5分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968504
求助须知:如何正确求助?哪些是违规求助? 3513278
关于积分的说明 11167234
捐赠科研通 3248660
什么是DOI,文献DOI怎么找? 1794386
邀请新用户注册赠送积分活动 875030
科研通“疑难数据库(出版商)”最低求助积分说明 804638