细胞生物学
生物
造血
干细胞
转录组
背主动脉
胚胎干细胞
血管母细胞
CXCR4型
造血干细胞
受体
基因
基因表达
遗传学
趋化因子
作者
Brandon Hadland,Barbara Varnum‐Finney,Stacey Dozono,Tessa Dignum,Cynthia Nourigat-Mckay,Adam M. Heck,Takashi Ishida,Dana L. Jackson,Tomer Itkin,Jason M. Butler,Shahin Rafii,Cole Trapnell,Irwin D. Bernstein
标识
DOI:10.1038/s41467-022-28781-z
摘要
Abstract Hematopoietic stem cells (HSCs) develop from hemogenic endothelium within embryonic arterial vessels such as the aorta of the aorta-gonad-mesonephros region (AGM). To identify the signals responsible for HSC formation, here we use single cell RNA-sequencing to simultaneously analyze the transcriptional profiles of AGM-derived cells transitioning from hemogenic endothelium to HSCs, and AGM-derived endothelial cells which provide signals sufficient to support HSC maturation and self-renewal. Pseudotemporal ordering reveals dynamics of gene expression during the hemogenic endothelium to HSC transition, identifying surface receptors specifically expressed on developing HSCs. Transcriptional profiling of niche endothelial cells identifies corresponding ligands, including those signaling to Notch receptors, VLA-4 integrin, and CXCR4, which, when integrated in an engineered platform, are sufficient to support the generation of engrafting HSCs. These studies provide a transcriptional map of the signaling interactions necessary for the development of HSCs and advance the goal of engineering HSCs for therapeutic applications.
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