麦胚凝集素
纳米探针
沙门氏菌
化学
量子点
荧光
细菌
微生物学
小麦胚芽
铜绿假单胞菌
材料科学
纳米技术
生物
生物化学
凝集素
物理
光学
遗传学
作者
Zhijie Tu,Xingsheng Yang,Hao Dong,Qing Yu,Shuai Zheng,Xiaodan Cheng,Chongwen Wang,Zhen Rong,Shengqi Wang
出处
期刊:Biosensors
[Multidisciplinary Digital Publishing Institute]
日期:2022-10-31
卷期号:12 (11): 942-942
被引量:18
摘要
Point-of-care testing methods for the rapid and sensitive screening of pathogenic bacteria are urgently needed because of the high number of outbreaks of microbial infections and foodborne diseases. In this study, we developed a highly sensitive and multiplex lateral flow assay (LFA) for the simultaneous detection of Pseudomonas aeruginosa and Salmonella typhimurium in complex samples by using wheat germ agglutinin (WGA)-modified magnetic quantum dots (Mag@QDs) as a universal detection nanoprobe. The Mag@QDs-WGA tag with a 200 nm Fe3O4 core and multiple QD-formed shell was introduced into the LFA biosensor for the universal capture of the two target bacteria and provided the dual amplification effect of fluorescence enhancement and magnetic enrichment for ultra-sensitivity detection. Meanwhile, two antibacterial antibodies were separately sprayed onto the two test lines of the LFA strip to ensure the specific identification of P. aeruginosa and S. typhimurium through one test. The proposed LFA exhibited excellent analytical performance, including high capture rate (>80%) to the target pathogens, low detection limit (<30 cells/mL), short testing time (<35 min), and good reproducibility (relative standard deviation < 10.4%). Given these merits, the Mag@QDs-WGA-based LFA has a great potential for the on-site and real-time diagnosis of bacterial samples.
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